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Thermo Scientific™ Thermus thermophilus DNA Ligase
Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.
Supplier: Thermo Scientific™ EL07981
Description
Thermo Scientific™ Thermus thermophilus DNA Ligase is a thermostable NAD-dependent DNA ligase that catalizes formation of phosphodiester bonds between 5`-phospate and 3`-hydroxyl termini in double-stranded DNA. Thermus thermophilus DNA Ligase is not active on RNA, single-stranded DNA, or blunt-ended DNA.
Features
- Increased specificity—high ligation temperature eliminates non-specific ligation
- Stabile at high temperatures with an optimum ligation temperature of 45°C to 65°C
Applications
Thermus thermophilus DNA ligase is recommended for applications requiring high ligation specificity, including:
- Single-stranded nick repair in double-stranded DNA
- Next-generation sequencing
- Rolling circle amplification
- Proximity ligation assay
Spécifications
| 50 U/μL | |
| 10X Thermus thermophilus DNA Ligase Buffer, 40 μL Store at -20°C. |
|
| No | |
| NAD-dependent DNA Ligase | |
| Liquid |
| Single-stranded nick repair, NGS, RCA, PLA | |
| DNA Ligase | |
| 1000 U | |
| Next-generation Sequencing, DNA Repair |
For Research Use Only. Not for use in diagnostic procedures.