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Invitrogen™ LR Clonase II for BaculoDirect™ Kits

Catalog No. 11791023
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11791023 10 reactions
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Catalog No. 11791023 Supplier Invitrogen™ Supplier No. 11791023
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Description

Eukaryotic system for high-level protein expression in insect cells

  • Combination of Gateway Technology with baculovirus expression makes BaculoDirect System fastest and easiest method for production of recombinant baculovirus

Cloning, Gateway Cloning, Gateway Recombination

Order Info

Shipping Conditions: Dry ice

Specifications

Content And Storage Store LR Clonase™ mix at -80°C. All components are guaranteed stable for 6 months if stored properly.
Shipping Condition Dry Ice
Enzyme LR Clonase
Quantity 10 reactions
Product Type LR Clonase II
Product Line BaculoDirect, Clonase, Gateway

Frequently Asked Questions (FAQs)

Can I use E. coli containing an F' plasmid, such as Top 10F', to propagate a vector with the ccdB gene?

While the F' plasmid does contain the ccdA gene that can inhibit or reduce the toxicity of the ccdB gene product, the ccdA expression level is likely to be too low, or inhibition may not be complete, and the bacteria would still be exposed to the ccdB gene product and thus not grow. Therefore, bacterial strains containing the F' plasmid are not recommended as hosts for propagation of ccdB containing vectors.

For propagation of Gateway vectors containing ccdB, we recommend the One Shot ccdB Survival 2 T1R Competent Cells (A10460), which were specifically designed for that purpose. However, please note that these cells are not validated for propagation of other ccdB-containing vectors like the older pZErO plasmids, and in most cases they are not expected to work due to very high levels of ccdB protein expressed in those vectors.

For Research Use Only. Not for use in diagnostic procedures.