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Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
Content And Storage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPOâ„¢-TA |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Shipping Condition | Dry Ice |
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Promoter | T7, T3 |
Content And Storage | Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPOâ„¢ |
Vector | TOPO Cloning Vectors |
Product Line | TOPOâ„¢ |
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Kanamycin (KanR), Zeocinâ„¢ (ZeoR) |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPOâ„¢ |
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Promoter | SP6 |
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Content And Storage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Format | Kit |
Product Type | PCR Cloning Kit |
Cloning Method | Blunt TOPOâ„¢ |
Bacterial or Yeast Strain | TOP10 |
Cell Type | Chemically Competent |
Used in suspension-adapted cells for transient protein expression. Gibcoâ„¢ pcDNAâ„¢3.4 TOPOâ„¢ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.
Enables rapid cloning of one or more genes into virtually any protein expression system
Enables rapid cloning of one or more genes into virtually any protein expression system
Invitrogenâ„¢ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.
Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Product Type | Cloning Kit |
Cloning Method | TOPOâ„¢-TA |
Product Line | TOPOâ„¢ |
Promoter | T7, SP6 |
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Content And Storage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Antibiotic Resistance Bacterial | Ampicillin (AmpR), Kanamycin (KanR) |
Format | Kit |
Cloning Method | TOPOâ„¢-TA |
Vector | TOPO-TA Vectors |
Product Line | TOPOâ„¢ |