PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.

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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

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Specifications

Content And Storage	Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Promoter	T7, T3
Content And Storage	Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Vector	TOPO Cloning Vectors
Product Line	TOPO™

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C.
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™
Bacterial or Yeast Strain	TOP10
Cell Type	Chemically Competent

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Pricing and Availability
Specifications

Promoter	SP6
Content And Storage	Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Kanamycin (KanR), Zeocin™ (ZeoR)
Format	Kit
Product Type	PCR Cloning Kit
Cloning Method	Blunt TOPO™

Invitrogen™ TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

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Invitrogen™ Bac-to-Bac™ HBM TOPO™ Cloning Kit

Bac-to-Bac™ HBM TOPO™ Cloning Kit

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Invitrogen™ Bac-to-Bac™ N-His TOPO™ Cloning Kit

Bac-to-Bac™ N-His TOPO™ Cloning Kit

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Invitrogen™ Bac-to-Bac™ C-His TOPO™ Cloning Kit

Bac-to-Bac™ C-His TOPO™ Cloning Kit

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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Pricing and Availability

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

Pricing and Availability

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, without competent cells

Pricing and Availability
Specifications

Antibiotic Resistance Bacterial	Ampicillin (AmpR), Kanamycin (KanR)
Format	Kit
Product Type	Cloning Kit
Cloning Method	TOPO™-TA
Product Line	TOPO™

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

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Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector, without competent cells

Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector

Pricing and Availability
Specifications

Promoter	T7
Content And Storage	TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	Cloning Kit
Cloning Method	TA Cloning
Bacterial or Yeast Strain	Not Included
For Use With (Application)	PCR Cloning
Vector	pCR2.1
Product Line	TA Cloning™

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1^R E. coli

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

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Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

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PCR Cloning Kits

PCR Cloning Kits

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