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Invitrogen™ Zero Blunt™ TOPO PCR Cloning Kit for Subcloning

Catalog No. K283020
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25 Reactions
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K283020 25 Reactions
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Catalog No. K283020 Supplier Invitrogen™ Supplier No. K283020
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Description

Includes

Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector; Salt solution; dNTPs; Control template; M13 forward and reverse primers; Sterile water; One Shot™ Chemically Competent or Electrocomp™ E. coli; S.O.C. medium; Supercoiled pUC19 control plasmid

Combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO vector to allow easy, high-efficiency cloning of blunt-end PCR products

  • Unique technology to minimize background
  • Get your clone: 95% efficiency
  • Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
  • Kanamycin and Zeocin resistance genes for your choice of selection
  • Convenient, validated restriction sites in MCS for further subcloning
  • Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time

Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Cloning, Methylation Analysis, PCR Cloning, RNAi, Epigenetics and Non-Coding RNA Research, Sequencing Bisulfite-Treated DNA

Specifications

Bacterial or Yeast Strain Mach1™
Cloning Method Blunt TOPO™
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
Format Kit
For Use With (Application) Chromatin Biology
Product Line One Shot
Product Type PCR Cloning Kit
Quantity 25 Reactions
Vector Blunt DNA Cloning Vectors

For Research Use Only. Not for use in diagnostic procedures.