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DNA Reagents

Chemical reagents and other products used for DNA-related techniques and processes such as PCR cloning, DNA sequencing, DNA cloning and mapping, mutagenesis, DNA synthesis, etc.; includes kits, columns, buffers, labelling technology, and others.
DNA Sequencing (221)
DNA Synthesis Reagents and Columns (107)
PCR Cloning Kits (104)
DNA Cloning and Mapping and Mutagenesis (83)
Plasmid DNA (11)

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1

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10F´
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
2

Applied Biosystems™ COLUMN WP T - 1UMOL  DFS Item

The 1.0μmol T Controlled Pore Glass (CPG) columns are suitable for the synthesis of any length oligonucleotide. These columns deliver high-quality oligonucleotides.

3

Applied Biosystems™ dGdmf, 1 μmol ABI 3900 HT Columns  DFS Item

The 1μmol dG(dmf) ABI 3900 HT columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. These supports are filled with polystryene, a material which has several advantages over the more conventional, controlled pore glass (CPG) filled columns.

4

Applied Biosystems™ T, 1.0 μmol 3900 Column  DFS Item

Each contains 200 1μmol T ABI 3900 HT columns.The 1μmol T ABI 3900 HT columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. These supports are filled with polystyrene, a material which has several advantages over the more conventional, controlled pore glass (CPG) filled columns.

5

Applied Biosystems™ T, 40 nmol ABI 3900 HT Columns  DFS Item

Each contains 200 40 nmol T ABI 3900 HT columns.The 40 nmol T ABI 3900 HT columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. These supports are filled with polystryene, a material which has several advantages over the more conventional, controlled pore glass (CPG) filled columns.

6

Applied Biosystems™ Tetrazole Acetonitrile  DFS Item

This tetrazole acetonitrile solution comes in Trap-Pak™ bags. Trap-Pak™ bags are tightly sealed membrane packets containing molecular sieves to efficiently adsorb water. Use Trap-Pak™ bags to reduce and maintain a low water content in acetonitrile and tetrazole (activator) solutions.

7

Applied Biosystems™ T, 0.2 μmol ABI 3900 HT Columns  DFS Item

Each contains 200 0.2μmol T ABI 3900 HT columns.The 0.2μmol T ABI 3900 HT columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. These supports are filled with polystyrene, a material which has several advantages over the more conventional, controlled pore glass (CPG) filled columns.

8

Applied Biosystems™ COL 200NMOL T LOW VOLUME  DFS Item

The T ABI LV200 columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. These supports are filled with polystyrene, a material which has several advantages over the more conventional, controlled pore glass (CPG) filled columns.

9

Applied Biosystems™ TFA Aminolink Phosphoramidite, 0.25 g  DFS Item

Each contains 0.25 g TFA Aminolink.TFA Aminolink is used for 5’ attachment of amine group during oligonucleotide synthesis.We offer several phosphoramidites that impart linkers for coupling other labels, and the means to add a phosphate during oligonucleotide synthesis. The label is treated as if it were simply another base: It is inserted at the appropriate place in the sequence and incorporated automatically.

10

Applied Biosystems™ CPG (Controlled Pore Glass) Synthesis Column, 1.0 μmol, 500 Angstrom Pore Size, T  DFS Item

The 1.0μmol T Controlled Pore Glass (CPG) columns are suitable for the synthesis of oligonucleotides<50 bases in length. These columns deliver high-quality oligonucleotides.

13

Honeywell Burdick & Jackson™ Activator Reagent, 0.6M ETT in Acetonitrile

BioSyn™, for DNA/RNA synthesis

CAS 75-05-8
Densité 0.8117 g/cm3
Type d’emballage Glass Bottle
14

Applied Biosystems™ Trichloroacetic Acid Dichloromethane, TCA DCM Solution  DFS Item

This Trichloroacetic acid (TCA)/Dichloromethane (DCM) solution comes in a convenient large volume for ABI 394 Nucleic Acid synthesizers, resulting in extended hands-free DNA synthesis. With these larger bottles attached your synthesizer can produce up to 40 average primer-length oligonucleotides with no bottle changes, reducing attended operation time by 50%.

15

Applied Biosystems™ TUB 60 DG(DMF)LV40NMOL COLUMNS  DFS Item

Contains 60 dG(dmf) ABI LV40™ columns.The dG(dmf) ABI LV40™ columns are fully assembled, low-cost columns that produce high-quality oligonucleotides. ABI LV40™ columns employ the advantages of polystyrene and a low volume column design to deliver high-quality oligonucleotides, while saving time and money.