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DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Invitrogen™ GeneArt™ Seamless Cloning and Assembly Kit  DFS Item

Enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction

2

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix  DFS Item

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

Shipping Condition Dry Ice
Content And Storage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
Workflow Step DNA Assembly
Number of Fragments Up to 15 fragments
Product Type Master Mix
Control Type Positive Control
Cloning Method Seamless cloning
For Use With (Application) Seamless Cloning & Genetic Assembly
Product Line GENEART
3

Invitrogen™ TrueTag™ Donor DNA Kit, YFP  DFS Item

The Invitrogen TrueTag Donor DNA Kit, YFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

4

Invitrogen™ TrueTag™ Donor DNA Kit, Myc  DFS Item

The Invitrogen TrueTag Donor DNA Kit, Myc, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

5

Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit  DFS Item

The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

6

Invitrogen™ GeneArt™ Seamless PLUS Cloning and Assembly Kit  DFS Item

The complete kit for simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute or less, room temperature reaction

8

Thermo Scientific™ phiX174 RF1 DNA

Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.

9

Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

10

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

11

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

12

Thermo Scientific™ pUC57 DNA

Perform cloning, sequencing of insert DNA, and generation of nested deletions with ExoIII using this double-stranded closed circular high copy plasmid DNA.

13

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.

14

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 2 (N-terminal His-tag/EK)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

15

Thermo Scientific™ aLICator LIC Cloning and Expression Set 2 (All-in-One/WQ)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.