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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

2

Gibco™ Collagenase, Type II, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen

3

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Type de produit Cell Culture Dissociation Reagent
Testé pour In vitro Bioassay
Concentration 1 X
Source Animal Origin
Conditions d’expédition Wet Ice
Avec additifs Phenol Red
Forme Liquid
Contenu et stockage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Sterilization Method Sterile-filtered
Stérilité Sterile-filtered
Classification Animal Origin
Gamme de produits Gibco
Type de cellule Mammalian Cells
Chélateurs No EDTA
Osmolalité 280 - 310 mOsm/kg
Durée de conservation 24 Months
4

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Type de produit Collagenase
Stérilité Non-sterile
Durée de conservation 24 Months
Conditions d’expédition Room Temperature
Forme Lyophilized
Contenu et stockage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
5

MilliporeSigma™ Enzyme Free Cell Dissociation Solution Hank's Based (1x)

Hank's based formulation of chelating agents and agents to stabilize their activity on cells

Formule Chelating and stabilizing agents
Contenu et stockage 4°C
6

Gibco™ Trypsin-EDTA (0.05%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

Sterilization Method Sterile-filtered
Type de produit Cell Culture Dissociation Reagent
Testé pour In vitro Bioassay
Stérilité Sterile-filtered
Classification Animal Origin
Gamme de produits Gibco
Type de cellule Mammalian Cells
Chélateurs EDTA
Osmolalité 270 - 310 mOsm/kg
Durée de conservation 24 Months
Source Animal Origin
Avec additifs Phenol Red,EDTA
Forme Liquid
7

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red  DFS Item
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Type de produit Cell Culture Dissociation Reagent
Testé pour In vitro Bioassay
Concentration 1 X
Source Animal origin-free
Conditions d’expédition Room Temperature
Forme Liquid
Type de culture Adherent Cell Culture
Remarques Room Temperature Stable
Stérilité Sterile-filtered
Classification Animal Origin-free
Gamme de produits Gibco
Sans additif No Phenol Red
Type de cellule Mammalian Cells
Chélateurs EDTA
Osmolalité Check and Record
Durée de conservation 24 Months
8

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

9

MilliporeSigma™ Chemicon™ Accumax™ Cell Detachment Solution

A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

10

Gibco™ Trypsin-EDTA (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Type de produit Cell Culture Dissociation Reagent
Testé pour In vitro Bioassay
Source Animal Origin
Avec additifs Phenol Red,EDTA
Forme Liquid
Contenu et stockage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Sterilization Method Sterile-filtered
Stérilité Sterile-filtered
Classification Animal Origin
Gamme de produits Gibco
Type de cellule Mammalian Cells
Chélateurs EDTA
Osmolalité 270 - 320 mOsm/kg
Durée de conservation 24 Months
11

Gibco™ Trypsin Neutralizer Solution  DFS Item
GREENER_CHOICE

Sterile, phosphate-buffered saline solution

12

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Type de produit Cell Culture Dissociation Reagent
Stérilité Sterile
pH 7 to 7.6
Sans additif No Calcium,No Magnesium,No Sodium Bicarbonate
Forme Liquid
Contenu et stockage -20°C
13

Invitrogen™ Accutase - Enzyme Cell Detachment Medium

Invitrogen™ Accutase Enzyme Cell Detachment Medium performs exceptionally well in detaching cells for analysis of cell surface markers and virus growth assay.

14

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

15

MilliporeSigma™ ESGRO Complete™ Accutase™ Cell Detachment Solution

a cell detachment solution of proteolytic and collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.