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  6. Gibco™ Trypsin (0.25%), phenol red

Gibco™ Trypsin (0.25%), phenol red
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Catalog No. 15050065
Encompass
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15050065 100 mL
15050057 500 mL
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Catalog No. 15050065 Supplier Gibco™ Supplier No. 15050065
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Description

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

  • Gibco™ Trypsin solutions are made from trypsin powder—an irradiated mixture of proteases derived from porcine pancreas
  • Due to its digestive strength it is widely used for cell dissociation, during routine cell culture passaging and primary tissue dissociation
  • This solution is formulated with phenol red indicator, and without EDTA
  • Gibco™ Trypsin solutions are tested for pH, osmolality, sterility and performance
  • Prior to manufacturing the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal and viral contamination, as well as multiple activity assays, ash analysis and moisture analysis
  • Lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency are all available
  • Solution should be stored at -5°C to -20°C

Order Info

Shipping Condition: Dry Ice

Compliance

Manufactured at a cGMP compliant facility that is registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards.

Specifications

Chelators No EDTA
Content And Storage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Cell Type Mammalian Cells
Classification Animal Origin
Concentration 1 X
Form Liquid
Product Type Cell Culture Dissociation Reagent
Sterility Sterile-filtered
Sterilization Method Sterile-filtered
With Additives Phenol Red
Osmolality 280 - 310 mOsm/kg
Product Line Gibco
Quantity 100 mL
Shipping Condition Wet Ice
Source Animal Origin
Tested For In vitro Bioassay
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Frequently Asked Questions (FAQs)

How long can I keep trypsin in the refrigerator after thawing?

Generally speaking, trypsin can be stored refrigerated for up to a week. However, this may vary depending on how often it is warmed up and used during that week. It may also depend on how tightly the cells are attached, because there will be some loss of activity each day the trypsin is not frozen.
Do you have a non-animal-origin enzyme for cell dissociation?

Yes, the TrypLE products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.
What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?

All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.

The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.

TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.

TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.

CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.

To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

What are the benefits of Gibco TrypLE reagent over trypsin?

TrypLE reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE reagent is room-temperature stable and ready to use when you need it. TrypLE cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.
What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Do you offer media without phenol red (phenol red-free media)?

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

For Research Use or Further Manufacturing. Not for use in diagnostic procedures.