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Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent

Catalog No. A1110501
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A1110501 100 mL
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Catalog No. A1110501 Supplier Gibco™ Supplier No. A1110501
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Description

StemPro Accutase is a gentle cell dissociation solution for routine detachment of cells from tissue culture plasticware or adhesion coated plasticware.

StemPro Accutase reagent is a gentle cell dissociation solution used for the detaching of cells while maintaining high cell viability as compared to animal-origin enzymes such as trypsin. It does not contain mammalian- or bacterial-derived products. Cell lines tested for StemPro Accutase reagent application include human embryonic stem cells, human mesenchymal stem cells, human neural stem cells, and primary cells.

StemPro Accutase Cell Dissociation Reagent is a cell detachment solution of proteolytic and collagenolytic enzymes. It is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including Geltrex, CELLStart, and polymers.

Maintains high cell viability

StemPro Accutase reagent performs well in detaching primary and stem cells while maintaining high cell viability compared to animal origin enzymes such as trypsin. Some downstream applications following treatment include analysis of cell surface markers, virus growth assay, cell proliferation, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Detaches various cell types

Cell lines tested for StemPro Accutase reagent application include human embryonic stem cells (hESCs), human mesenchymal stem cells (hMSCs), human neural stem cells (hNSCs), and primary cells including macrophages, fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, and established cell lines such as adherent CHO and BHK cells and 293 cells.

Specifications

Content And Storage Store in freezer (-5 to -20°C) and protect from light.
Cell Type Stem Cells
Classification Serum-free
Culture Type Adherent Cell Culture
Form Frozen
Product Type Cell Culture Dissociation Reagent
Serum Level Serum-Free
Inorganic Salts Sodium Phosphate
pH 6 to 8
Product Line Gibco, StemPro
Purity or Quality Grade Research Grade
Quantity 100 mL
Shipping Condition Dry Ice
Source Serum-Free
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Frequently Asked Questions (FAQs)

Do I need to worry about over-dissociating my cells with StemPro Accutase Cell Dissociation Reagent?

No. However, although StemPro Accutase is gentle on cells, the optimal time for dissociation should be empirically determined for your specific cell type and application.
Do I need to dilute StemPro Accutase Cell Dissociation Reagent before using it?

No. StemPro Accutase is supplied as a convenient, ready to use reagent. No dilution is required prior to use.
With StemPro Accutase Cell Dissociation Reagent, do I need to stop the dissociation reaction with serum?

No. StemPro Accutase is gentle enough that only dilution of the reagent with DPBS or media is required to stop the dissociation activity.

How long can I store StemPro Accutase Cell Dissociation Reagent at 2 to 8 degrees C?

We recommend storing StemPro Accutase Cell Dissociation Reagent at -5 to -20 degrees C, protected from light. We do not recommend subjecting it to more than 3-4 freeze-thaws. When stored at 2 to 8 degrees C, the reagent is stable for up to 2 years.
I accidentally thawed the StemPro Accutase Cell Dissociation Reagent at 37 degrees C. Can I still use it?

Yes. If StemPro Accutase is exposed to 37 degrees C just until complete thawing is achieved, it still can be used. However, this could result in decreased enzyme activity and it would take more time to get full dissociation. If you observe this, consider starting with a new bottle. Please note that if a bottle of StemPro Accutase is kept at 37 degrees C for more than one hour, it will lose its activity.

My StemPro Accutase Cell Dissociation Reagent arrived completely thawed. Can I still use it?

Yes, as long as the StemPro Accutase is still cool to the touch, it should be okay to use.

My StemPro Accutase Cell Dissociation Reagent arrived partially thawed, can I still use it?

Yes. We recommend thawing it at room temperature or overnight at 4 degrees C to get complete thawing. Otherwise, place in the freezer and refreeze.

The frozen bottle of StemPro Accutase Cell Dissociation Reagent shows uneven color distribution and upon thawing, it has layered color distribution. Is this appearance normal?

Yes. During the freezing procedure or shipping, uneven color can be observed. However, this will not compromise the activity or performance of the enzyme. Just make sure to mix the solution by inverting the tube or bottle to get an even color before use.
Can I use StemPro Accutase Cell Dissociation Reagent to dissociate non-adherent cells?

Yes. StemPro Accutase has been tested to dissociate spheres of neural progenitors (neurospheres).
What are the target cell lines that StemPro Accutase Cell Dissociation Reagent can be used for?

We recommend using StemPro Accutase wherever gentle and efficient dissociation is needed. We have also validated StemPro Accutase for use with hESC grown in StemPro hESC SFM and for NSC grown in StemPro NSC SFM. All the tests resulted in great recovery of cells without losing their properties and while maintaining good viability. In addition, the manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/5029_A1110501_Accutase.pdf) lists examples of cell lines where the application has been validated and published.

Which dissociation reagent should I use with the Gibco PSC Definitive Endoderm Induction Kit?

We recommend using EDTA for passaging PSCs. For seeding cells for definitive endoderm induction, we recommend using StemPro Accutase Cell Dissociation Reagent for small clumps and Gibco TrypLE Enzyme for singularized cells.
How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).
How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).
What are ES cells?

Embryonic stem (ES) cells are derived from the early mammalian embryo and are capable of unlimited, undifferentiated proliferation in vitro while maintaining their potential to differentiate into a wide of range of adult tissues including germ cells. The pluripotency of the ES cells is normally demonstrated in vitro by inducing ES cells to differentiate into embryoid bodies and checking lineage-specific markers for differentiated cells in three body layers (endo, meso, and ectoderm), or injecting them into immunodeficient mice and determining the cell types produced in the teratomas.

What reagents do you offer for cell dissociation, and what are the main differences between them?

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

What can be used to detach cells from plates when coated with a thin layer of collagen I?

Enzymes that can be used include: Gibco TrypLE Express Enzyme, Gibco TrypLE Select Enzyme, or Gibco StemPro Accutase Cell Dissociation Reagent.

What other dissociation reagents have been tested with Gibco Essential 8 Flex Medium?

Gibco TrypLE Select Enzyme (Cat. No. 12563011) and Gibco StemPro Accutase Cell Dissociation Reagent (Cat. No A1110501) have been tested with Essential 8 Flex Medium.

For Research Use Only. Not for use in diagnostic procedures.