Real Time PCR Master Mixes
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Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and 1-Step RT-dPCR Master Mix
A key component of the QuantStudio Absolute Q Digital PCR System which is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated sample separation.
Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)
Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.
Thermo Scientific™ Maxima Probe qPCR Master Mixes
Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.
Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial
Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.
| Polymérase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon |
| Vitesse de réaction | Standard |
| Format | Tube |
| Type de produit | qPCR Master Mix |
| Gamme de produits | Maxima |
| Type d’échantillon | DNA, dsDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.
| Thermostabilité | Not Applicable |
|---|---|
| Capacité multiplex | Up to 4-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | None |
| Type d’échantillon | RNA, DNA |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| Hot Start | Built-In Hot Start |
| Spécificité cible | RNA only, Both RNA and DNA |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
| Thermostabilité | 8 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 5-plex |
| GC-Rich PCR Performance | Medium |
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | ROX (Pre-mixed) |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| Forme | Liquid |
| Contenu et stockage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Polymérase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Hot Start | Antibody-based |
| Gamme de produits | TaqPath |
| Comprend | dUTP |
| Durée de conservation | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Spécificité cible | RNA only, Both RNA and DNA |
Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG
Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
| Vitesse de réaction | Standard |
| Type de produit | Quantitative PCR SuperMix-UDG |
| Gamme de produits | Platinum |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
Invitrogen™ EXPRESS qPCR Supermix, universal
EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for probe-based qPCR assays.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast or Standard |
| Format | Tube |
| Type de produit | Express qPCR Supermix |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
| Thermostabilité | Not Applicable |
|---|---|
| Capacité multiplex | Up to 3-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | ROX (Pre-mixed) |
| Type d’échantillon | RNA, cDNA, DNA |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| À utiliser avec (application) | Microbial Detection,DNA Quantification,Presence/Absence |
| Forme | Liquid |
| Polymérase | Fast DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Gamme de produits | TaqPath |
| Sensibilité | Down to 10 copies |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | RNA only, Both RNA and DNA |
| Transcriptase inverse | M-MLV |
Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
| Polymérase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast |
| Durée d’activation | 5 min. |
| Hot Start | Built-in Hot Start |
| Type de produit final | Mastermix |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 2X |
| Compatibilité | TaqMan™ |
| Matériau de départ | DNA/RNA |
| À utiliser avec (application) | Animal Health |
| Marqueur ou colorant | ROX |
| Forme | Liquid |
Invitrogen™ EXPRESS qPCR Supermix, with premixed ROX
EXPRESS qPCR SuperMix kits are compatible with fast-cycling instruments for your probe-based qPCR assays.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, StepOne™, Fast Mode, StepOne™, Standard Mode |
| Vitesse de réaction | Fast or Standard |
| Format | Tube |
| Type de produit | Express qPCR Supermix |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| À utiliser avec (application) | Gene Expression |
| Contenu et stockage | EXPRESS qPCR SuperMix kits are provided as a 2X qPCR SuperMix with ROX premixed or in a separate tube. Store at -20°C upon receipt. Once thawed, the EXPRESS qPCR SuperMix can be stored for up to 1 month at +4·C. Product is guaranteedstable for 6 months when properly stored. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| À utiliser avec (équipement) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Vitesse de réaction | Standard |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | LD (Low DNA) |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Polymérase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
| Gamme de produits | SYBR |
| Marqueur ou colorant | SYBR Green |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
| Polymérase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | SYBR |
| Vitesse de réaction | Standard |
| Format | Tube |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Gamme de produits | SYBR |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Marqueur ou colorant | SYBR Green |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| À utiliser avec (équipement) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Vitesse de réaction | Fast |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | UP (Ultra Pure) |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Polymérase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
| Gamme de produits | SYBR |
| Marqueur ou colorant | SYBR Green |
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| Vitesse de réaction | Fast or Standard |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | UP (Ultra Pure) |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Forme | Liquid |
| Contenu et stockage | The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems. |
| Polymérase | AmpliTaq DNA Polymerase |
| Format | Vial |
| Suffisant pour | 100 Reactions of 20 μL |
| Hot Start | Built-In Hot Start |
| Gamme de produits | SYBR |
| Marqueur ou colorant | SYBR GreenER |