Thermo Scientific™ AmpSure™ Universal SYBR Green qPCR Master Mix

Features of Thermo Scientific AmpSure Universal SYBR Green qPCR Master Mix include:

• Universal compatibility with major real-time PCR instruments and commonly used reverse transcriptase (RT) enzymes
• Reliable qPCR performance for routine gene expression analysis and target quantification
• Sensitive and specific target detection with clean melt curve profiles
• Wide linear dynamic range supporting accurate quantitative PCR measurements
• Compatible with standard and fast qPCR cycling protocols

Universal compatibility across instruments and workflows

AmpSure Universal SYBR Green qPCR Master Mix is designed to work with a broad range of real-time PCR instruments, providing flexibility for laboratories operating multiple platforms. Universal compatibility helps simplify assay transfer and supports consistent performance across diverse qPCR workflows. The mix is also compatible with a variety of reverse transcriptase enzymes, allowing seamless integration into existing cDNA synthesis and gene expression workflows.

Reliable performance for everyday qPCR

Designed as a dependable qPCR workhorse, AmpSure Universal SYBR Green qPCR Master Mix combines an optimized formulation with antibody-mediated hot-start technology to support sensitive and specific target detection while helping reduce non-specific amplification. Consistent performance across a wide range of targets and assay conditions provides confidence in routine real-time PCR experiments.

Wide dynamic range for accurate quantification

Reliable amplification across a broad range of target concentrations is critical for accurate quantification of both low- and high-abundance targets in gene expression studies. AmpSure Universal SYBR Green qPCR Master Mix demonstrates a six-log linear dynamic range when amplifying the FN1 (Fibronectin 1) gene from universal human reference (UHR) cDNA. Amplification curves remain tightly clustered between replicates, supporting highly reproducible quantitative PCR measurements. In testing on the Applied Biosystems™ QuantStudio™ 5 Real-Time PCR System, the master mix demonstrated 100.9% PCR efficiency with an R² value of 0.998, supporting accurate quantification of both low- and high-abundance targets.

Specific amplification with clean melt curves

Specific amplification is essential for confidence in SYBR Green qPCR experiments. When amplifying PRTN3 (Proteinase 3) and FN1 targets from UHR cDNA, AmpSure Universal SYBR Green qPCR Master Mix generated a single melt curve peak, demonstrating highly specific amplification. In comparative testing, competitor master mixes produced multiple melt curve peaks indicative of non-specific amplification products. Clean melt curve analysis helps improve confidence in target detection and gene expression results while minimizing ambiguity in data interpretation.

Applications

AmpSure Universal SYBR Green qPCR Master Mix is suitable for:

• Gene expression analysis
• NGS library quantification
• Target validation
• cDNA target detection
• Genomic DNA target detection

Additional features

• Compatible with standard and fast cycling protocols
• Contains uracil-DNA glycosylase (UDG) and dUTP to help reduce carryover contamination
• Universal SYBR Green chemistry for routine real-time PCR applications

Designed to provide dependable, cost-effective qPCR performance across a broad range of workflows