accessibility menu, dialog, popup

UserName

Real Time PCR Master Mixes

Real-time PCR master mixes are batch mixtures of PCR reagents at optimal concentrations that can be prepared and divided among multiple PCR tubes/plates. They reduce pipetting and risk of contamination, save time, and limit possible mixing errors.
Detection Method 
  • (4)
  • (128)
  • (106)
PCR Method 
  • (1)
  • (31)
  • (14)
  • (5)
  • (16)
  • (13)
  • (204)
For Use With (Application) 
  • (7)
  • (28)
  • (6)
  • (3)
  • (195)
  • (62)
  • (53)
  • (18)
  • (1)
  • (2)
  • (28)
  • (4)
  • (19)
  • (39)
  • (2)
Reaction Speed 
  • (2)
  • (36)
  • (97)
  • (101)
GC-Rich PCR Performance 
  • (166)
  • (1)
  • (7)

Filtered Search Results

Products from some of our suppliers do not display in filtered search results. Please clear all filters to see these products.

Narrow Results

Narrow Results

category

special offers

  • (1)
  • (113)

brands

  • (159)
  • (10)
  • (25)
  • (3)
  • (1)
  • (41)

Detection Method

  • (4)
  • (128)
  • (106)

PCR Method

  • (1)
  • (31)
  • (14)
  • (5)
  • (16)
  • (13)
  • (204)

For Use With (Application)

  • (7)
  • (28)
  • (6)
  • (3)
  • (195)
  • (62)
  • (53)
  • (18)
  • (1)
  • (2)
  • (28)
  • (4)
  • (19)
  • (39)
  • (2)

Reaction Speed

  • (2)
  • (36)
  • (97)
  • (101)

GC-Rich PCR Performance

  • (166)
  • (1)
  • (7)

Concentration

  • (150)
  • (4)
  • (2)
  • (11)
  • (2)

Form

  • (1)
  • (4)
  • (64)

Label or Dye

  • (3)
  • (3)
  • (7)
  • (6)
  • (25)
  • (14)
  • (4)

Search Within Results
Keyword Search:
1 – 15 of 76 results
1

Applied Biosystems™ TaqMan™ Environmental Master Mix 2.0  DFS Item

Accurate, real-time PCR-based pathogen detection in the presence of high levels of inhibitors.

Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,Pathogen Detection,Virus Detection
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOne™, Standard Mode
Sample Type Environmental Sample, Food
Reaction Speed Standard
Hot Start Built-In Hot Start
Product Line TaqMan
2
Promotions Available

Applied Biosystems™ SYBR™ Green Universal Master Mix  DFS Item

Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
Label or Dye SYBR Green
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line SYBR
Detection Method SYBR
3
Promotions Available

Applied Biosystems™ TaqMan™ Universal PCR Master Mix  DFS Item

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Product Type Universal PCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Product Line TaqMan
Detection Method Primer-probe
4
Promotions Available

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR  DFS Item

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Shipping Condition Wet Ice
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Dual-Lock Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Medium
Target Specificity cDNA or gDNA only
Product Line PowerUP, SYBR
5
Promotions Available

Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR  DFS Item

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up.

Shipping Condition Dry Ice
Content And Storage • PowerTrack SYBR Green Master Mix
• 40X Yellow Sample Buffer

Store at -20±5°C in the dark.
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection
PCR Method qPCR
Multiplex Capability Single-plex
Detection Method SYBR
Includes dUTP, UNG/UDG
Thermostability 8 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Format Tube
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, QuantStudio 12K Flex, Bio-Rad CFX Connect, Bio-Rad CFX384 Touch, Bio-Rad CFX96 Touch, BioRad CFX96, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, ViiA 7 System, StepOne System, MJ Chromo4, MJ Opticon, StepOnePlus System, Roche LightCycler 480, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Product Line Applied Biosystems
6

Applied Biosystems™ SYBR™ Select Master Mix  DFS Item

Cost-effective solution for real-time PCR applications.

GC-Rich PCR Performance High
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Sufficient For 100 Reactions of 20 μL
Product Line SYBR
7

Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)

Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.

8
Promotions Available

Applied Biosystems™ TaqPath™ ProAmp™ Master Mix  DFS Item

TaqPath ProAmp Master Mix is for high-throughput genotyping and copy number variation protocols requiring uncompromising reliability and accuracy, even in the presence of inhibitors commonly found in clinical samples.

Shipping Condition Wet Ice
Content And Storage Store at 2°C to 8°C in the dark.
For Use With (Application) Genotyping
PCR Method qPCR
Multiplex Capability 4-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Polymerase Taq DNA Polymerase
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 7 Flex, ViiA 7 System, ViiA 7 Dx, QuantStudio Dx, StepOnePlus System, StepOne System
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Very High
Target Specificity cDNA or gDNA only
Product Line TaqPath
9

Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and Absolute Q™ Universal DNA dPCR Master Mix

Eliminate the need to design custom assays and choose from over 20 million predesigned assays. Includes the Absolute Q MAP16 plate kit plus the Absolute Q Universal DNA dPCR master mix which is compatible with a wide range of applications and difficult targets.

10
Promotions Available

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Shipping Condition Dry Ice
GC-Rich PCR Performance Medium
Content And Storage Store at -15°C to -25°C.
Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form Liquid
Concentration 4X
For Use With (Application) Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence
PCR Method 1-step RT-qPCR
Multiplex Capability Up to 5-plex
Detection Method Primer-probe
Includes dUTP
Thermostability 8 hr., Assembled Reaction
Polymerase Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format Single-Tube
Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity RNA only, Both RNA and DNA
Hot Start Antibody-based
Shelf Life Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line TaqPath
11

Thermo Scientific™ AmpSure™ Universal SYBR Green qPCR Master Mix

Thermo Scientific™ AmpSure™ Universal SYBR Green qPCR Master Mix is a 2X, ready-to-use master mix for dye-based real-time PCR (qPCR) applications.

12
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG  DFS Item

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
13
Promotions Available

Applied Biosystems™ TaqPath™ qPCR Master Mix, CG  DFS Item

TaqPath qPCR Master Mix, CG, is a 2X formulation designed for gene expression and miRNA analysis. It contains thermostable Fast DNA polymerase, uracil-N-glycosylase (UNG), dNTPs with dUTP, ROX dye (passive reference), and optimized buffer components for maximum robustness and reproducibility.

Content And Storage Store at 2°C to 8°C in the dark.
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression,Microbial Detection,DNA Quantification,Presence/Absence,miRNA analysis
PCR Method qPCR
Multiplex Capability Duplex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 48 hr., Assembled Reaction
Polymerase Fast DNA Polymerase
Sensitivity Down to 10 copies
Format Bottle
Product Type qPCR Master Mix
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) QuantStudio Dx, 7500 Fast Dx, Roche LightCycler PRO System, Bio-Rad CFX Opus 96 Real-Time PCR System
Sample Type cDNA, DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) High
Target Specificity cDNA or gDNA only
Hot Start Built-In Hot Start
Product Line TaqPath
14
Promotions Available

Applied Biosystems™ TaqMan™ Multiplex Master Mix  DFS Item

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).

Polymerase AmpliTaq Fast DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 2 X
Product Type Multiplex Master Mix
Sample Type DNA
Reaction Speed Fast
Concentration 2X
For Use With (Application) Gene Expression,Virus Detection
PCR Method Multiplex qPCR
Product Line TaqMan
Detection Method Primer-probe
15

Applied Biosystems™ TaqPath™ BactoPure™ Microbial Detection Master Mix  DFS Item

TaqPath BactoPure Microbial Detection Master Mix is optimized for rapid low-level microbial detection even in the presence of PCR inhibitors.

Content And Storage Contains 2X TaqPath BactoPure Microbial Detection Master Mix that includes:
• Hot-start AmpliTaq Fast DNA Polymerase
• Heat-labile uracil-DNA glycosylase (UNG)
• dNTPs (with dUTP)
• ROX dye (passive reference)
• Optimized buffer

Store at 2°C to 8°C in the dark.
For Use With (Application) Microbial Detection
PCR Method qPCR
Multiplex Capability Up to 3-plex
Detection Method Primer-probe
Includes dUTP, UNG/UDG
Thermostability 72 hr., Assembled Reaction
Sensitivity Down to 1 copy
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio12k Flex Dx, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio 5 Dx, QuantStudio 6 Pro, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 7 Pro, QuantStudio 7 Pro Dx, ViiA™ 7 System, ViiA 7 Dx, QuantStudio™ Dx, StepOnePlus™ System, 7500 Fast Dx System, StepOne™ System
Sample Type DNA
Reaction Speed Fast or Standard
Inhibitor Tolerance (Simple) Very High
Target Specificity cDNA or gDNA only
Product Line TaqPath