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Immersion oil is a clear, viscous liquid with optimized refractive properties, specifically modified to closely approximate the refractive index (RI) of glass (ne = 1.5). It is used in conjunction with an objective lens to enhance the resolving power.
Eliminate light beam deflection to considerably increase the effectiveness of the lens. Immersion Oil for Microscopy is used with immersion objectives and is located between the specimen and the microscope lens. The refractive index is similar to that of glass.