ELISA Substrates and Reagents
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Invitrogen™ eBioscience™ TMB Solution (1X)
Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.
Invitrogen™ Super AquaBlue ELISA Substrate
Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.
Invitrogen™ Assay Buffer (10X)
Used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix™ substrate conjugates.
Invitrogen™ cAMP-Screen™ Wash Buffer
For use with the cAMP-Screen or cAMP-Screen Direct™ Immunoassay system.
Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use)
This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.
Thermo Scientific™ ELISA Carbonate Coating Buffer
For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.
Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ ELISA Buffer Kit
Concentrated
Invitrogen™ ELISA-Light™ Immunoassay System with CDP-Star™ and Emerald-II™ Substrate/Enhancer Solution
Ultra-sensitive detection of antigens or antibodies in multiple immunoassay formats employing an alkaline phosphatase enzyme label.
Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate
HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.