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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen
2

MilliporeSigma™ Calbiochem™ ImmunoPen™

Useful in immunostaining techniques on paraffin, frozen sections cytology preparations.

3

Thermo Scientific™ Pierce™ Western Blot Signal Enhancer

Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot detection signal with this two-reagent blot conditioning system.

4

MilliporeSigma™ Calbiochem™ SeaBlock™ Reagent, Salmon Plasma

Ready to use blocking reagent for western blotting immunoassays.

5

Invitrogen™ iBind PLUS Solution Kit

The iBind PLUS Solution Kit increases signal sensitivity, requires less antibody per run, and simplifies western blotting immunoprocessing when used with the iBind Western System. The iBind PLUS Solution Kit comes with two ready to use solutions, iBind PLUS Solution and iBind PLUS Antibody Diluent.

Product Type Western Blot Kit
6

Invitrogen™ XCell II™ Blot Module  DFS Item

Easy transfer of proteins or nucleic acids from mini-gels to membranes

7

Thermo Scientific Chemicals Nitrocellulose stripping buffer (10X)

Form Liquid
Product Type Western Blot Stripping Buffer
Concentration 10X
8

MilliporeSigma™ Calbiochem™ MOWIOL™ 4-88 Reagent

Used for mounting cells on slides for detection of cell-surface antigens.

9

MilliporeSigma™ ReBlot Plus Kit and Reagents

Blots can be stripped of antibodies in approximately 15 minutes at room temperature

For Use With (Application) Western Blot
10

Thermo Scientific™ Restore™ Western Blot Stripping Buffer

Safely and effectively remove primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Form Liquid
Product Line Restore
11

Thermo Scientific™ Restore™ PLUS Western Blot Stripping Buffer

Remove high-affinity antibodies from membranes so they can be reprobed and detected with chemiluminescent substrates.

Form Liquid
Product Line Restore
12

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Form Liquid
Product Line Restore
13

Thermo Scientific™ TMB Substrate Solution

A ready-to-use substrate system containing 3.3', 5.5'-tetramethylbenzidine (TMB)

14

Invitrogen™ Novex™ Tris-Glycine SDS Buffer Kit  DFS Item

Made with high-purity reagents and are strictly quality controlled

15

Invitrogen™ Bolt™ Antioxidant  DFS Item

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis