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Invitrogen™ Tris-Glycine Transfer Buffer (25X)

Catalog No. LC3675
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LC3675 500 mL
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Catalog No. LC3675 Supplier Invitrogen™ Supplier No. LC3675
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Description

Requires

The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.

Made with high-purity reagents and are strictly quality controlled

Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer applications using Tris-Glycine gels. Premixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results.

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting

Order Info

Shipping Condition: Room Temperature

Specifications

Product Line Novex
Gel Compatibility Novex Tris-Glycine Gel
Membrane Compatibility Nitrocellulose, PVDF, Nylon
Product Type Transfer Buffer
Content And Storage Store at room temperature.
Concentration 25X
Gel Type Tris-Glycine Gel
Shipping Condition Room Temperature
Quantity 500 mL

Frequently Asked Questions (FAQs)

What type of transfer buffer should be used with Invitrogen Tricine gels?

For non-sequencing applications, any transfer buffer used with Tris-Glycine gels can be used with Tricine gels including Tris-Glycine transfer buffer. For sequencing applications, the buffer should be chemically compatible with sequencing protocols. Non-glycine based transfer buffers such as the NuPAGE Transfer buffer, 1/2X TBE Transfer buffer, or CAPS Buffer can be used for N-terminal sequencing . Generally, a pH which is close to neutral is desirable to maintain gel and protein stability. High current should be avoided because it can lead to heat generation and instability.
When blotting with the XCell II Blot Module, what would happen if I filled the outer buffer chamber with transfer buffer instead of water?

This is perfectly acceptable with the XCell II Blot Module. The liquid in the outer buffer chamber only serves as a coolant or heat sink. The reason why water is recommended is because it is a less expensive alternative.
The pH of my transfer buffer deviates from the recommended value by 0.2 units. Can I still use the buffer?

We recommend discarding the buffer and remaking it after rechecking the reagents and the water purity. We do not recommend adjusting the pH with acid or base, as this will increase the conductivity of the buffer and result in higher current during the transfer.

For Research Use Only. Not for use in diagnostic procedures.