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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 49 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
3

Thermo Scientific™ ELISA Wash Buffer (30X)

Concentrated wash buffer for efficient use of storage in the lab

4

Invitrogen™ eBioscience™ TMB Solution (1X)

Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.

5

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

6

Invitrogen™ ELISA/ELISPOT Coating Buffer Powder

Designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.

7

Invitrogen™ eBioscience™ ELISA Wash Buffer, 1L packets

Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.

8

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

9

Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

10

Thermo Scientific™ SuperSignal™ ELISA Femto Substrate

HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.

Content And Storage Stable for one year at 4°C or six months at room temperature. Product is shipped at ambient temperature.
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
11

Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

12

Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)

Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.

13

Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)

Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.

14

Thermo Scientific™ SuperSignal™ ELISA Pico Chemiluminescent Substrate

HRP substrate optimized to generate an intense light signal and provide exception performance in luminometer-based assays.

Content And Storage Upon receipt store product at room temperature. Product shipped at ambient temperature.Introduction
Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Product Line SuperSignal
Detection Method Chemiluminescence
15

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.