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1 – 15 of 73 results
1
Promotions Available

Invitrogen™ SuperScript™ IV CellsDirect™ cDNA Synthesis Kit
GREENER_CHOICE

The SuperScript IV CellsDirect cDNA Synthesis Kit is optimized for the synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating RNA. Cell lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in PCR and qPCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Green Features Less Hazardous, Less Waste
For Use With (Application) RT-PCR,Real Time PCR (qPCR)
Format Kit
Reaction Format Premixed Components
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material Cells
2
Promotions Available

Invitrogen™ SuperScript™ IV VILO™ Master Mix

SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Quantity Each
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
3
Promotions Available

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, without competent cells

Promoter SP6
Content And Storage Box 1:
• Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
Antibiotic Resistance Bacterial Kanamycin (KanR), Zeocin™ (ZeoR)
Format Kit
Product Type PCR Cloning Kit
Cloning Method Blunt TOPO™
4
Promotions Available

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome No
5
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Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.

6
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Invitrogen™ Platinum™ Taq DNA Polymerase

Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.

Polymerase Platinum Taq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Hot Start Built-In Hot Start
Overhang 3'-A
Starting Material DNA
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
7
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Invitrogen™ UltraPure™ DNase/RNase-Free Distilled Water
GREENER_CHOICE

Designed for use in all molecular biology applications

Treatment(s) Not DEPC-Treated
Purification Method Membrane-Filtered
Packaging Bottle
Grade Molecular Biology
Product Line UltraPure
8
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Invitrogen™ dNTP Set (100 mM)

Invitrogen 100 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water. Each nucleotide is at a concentration of 100 mM.

Shipping Condition Approved for shipment on Wet or Dry Ice
Form Liquid
Label or Dye Unlabeled
Concentration 100 mM
For Use With (Application) Standard PCR,RT-PCR,Real Time PCR (qPCR)
9
Promotions Available

Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System

Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.

GC-Rich PCR Performance High
Format 1-step RT-PCR Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 13 kb
Reaction Format Master Mix
Reagent Type Reverse Transcription
Hot Start Built-in Hot Start
Optimal Reaction Temperature 50°C to 65°C
Technique 1-step RT-PCR
Starting Material RNA
PCR Method 1-step RT-PCR
Multiplex Capability Yes
Detection Method Gel Electrophoresis
10
Promotions Available

Invitrogen™ Platinum™ SuperFi II DNA Polymerase

Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.

Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
11
Promotions Available

Invitrogen™ Platinum™ SuperFi II PCR Master Mixes

Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.

Shipping Condition Dry Ice
Polymerase Platinum SuperFi II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
12
Promotions Available

Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Format Tube
Size (Final Product) 5 kb or less
Hot Start Built-In Hot Start
Concentration 2X
For Use With (Application) Hot-start PCR,Hot-start PCR
13
Promotions Available

Invitrogen™ SuperScript™ IV Reverse Transcriptase

SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript IV
Final Product Type First-Strand cDNA
Concentration 200 U/μL
Format Stand-alone Enzyme
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
14
Promotions Available

Invitrogen™ SuperScript™ IV First-Strand Synthesis System

The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript IV
Size (Final Product) Up to 12.3 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 10 min.
Reagent Type Reverse Transcription
Quantity Each
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
15
Promotions Available

Thermo Scientific™ Phusion U Multiplex PCR Master Mixes

Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
Fidelity (vs. Taq) 25X
Size (Final Product) 2.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Multiplex PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X