High Fidelity PCR Reagents and Kits

High Fidelity PCR Reagents and Kits

PCR products designed to produce large yields of accurately-replicated target DNA by utilizing a DNA polymerase with a low error rate. Includes master mixes, DNA polymerases, kits, and reagents.
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Hot Start 
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Hot Start

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1 – 30 of 33 results
1

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/µL)

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

GC-Rich PCR Performance High
Format Stand-alone enzyme
Fidelity (vs. Taq) 52 X
Product Type High-Fidelity DNA Polymerase
Size (Final Product) 20 kb or Less
Reaction Format Standalone
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2 U/µL
For Use With (Application) PCR Amplification
Starting Material DNA
Product Line Phusion
2

Thermo Scientific™ Phusion Green Hot Start II High-Fidelity DNA Polymerase (2 U/µL)

The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5 mM MgCl2 in the final 1X concentration.Store at -20°C.
Fidelity (vs. Taq) 52 X
Overhang Blunt
Concentration 2 U/µL
Includes Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution
Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 20 kb or Less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
3

Thermo Scientific™ Phusion Site-Directed Mutagenesis Kit

Generate mutations with this fast and reliable system.

4

Thermo Scientific™ Phusion Hot Start II DNA Polymerase (2 U/µL)

Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance.

Polymerase Phusion Hot Start II DNA Polymerase
GC-Rich PCR Performance High
Format Stand-alone enzyme
Fidelity (vs. Taq) 52 X
Size (Final Product) 20 kb or Less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/µL
For Use With (Application) PCR Amplification
Starting Material DNA
Product Line Phusion
5

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF Buffer

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 52 X
Purity DHPLC
Overhang Blunt
Concentration 2X
For Use With (Application) PCR Amplification
Polymerase Phusion High-Fidelity DNA Polymerase
Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Size (Final Product) 20 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start No
Starting Material DNA
Product Line Phusion
6

Thermo Scientific™ Phusion U Multiplex PCR Master Mix

High performance multiplex PCR master mix for fast and reliable simultaneous amplification of multiple targets

GC-Rich PCR Performance Medium
Content And Storage Phusion U Multiplex PCR Master Mix includes:
• Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 µM of each dATP, dTTP, dCTP and dGTP
The master mix is supplied with nuclease-free water.
Store at -20°C.
Fidelity (vs. Taq) 25X
Overhang Blunt
Concentration 2X
Polymerase Phusion U Hot Start DNA Polymerase
Format Tube
Product Type Multiplex PCR Master Mix
Size (Final Product) 2.5 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
7

Thermo Scientific™ Phusion Green Hot Start II High-Fidelity PCR Master Mix

The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.

GC-Rich PCR Performance Medium
Content And Storage Includes:

• 2X Phusion Green Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.
Fidelity (vs. Taq) 52 X
Overhang Blunt
Concentration 2X
For Use With (Application) PCR Amplification
Includes 2X Phusion Green Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO
Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase
Format SuperMix or Master Mix
Product Type Hot Start High-Fidelity PCR Master Mix
Size (Final Product) 20 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
8

Thermo Scientific™ Phusion High-Fidelity PCR Kit

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion DNA Polymerase (2 U/µL)
• 5X Phusion GC Buffer
• 5X Phusion HF Buffer
• dNTP mix (10 mM each)
• MgCl2 solution (50 mM)
• Control template (λ DNA)
• 1.3 kb primers (4 µM each)
• 10 kb primers (4 µM each)
• DNA size standard
• DMSO

Store at -20°C.
Fidelity (vs. Taq) 52 X
Overhang Blunt
For Use With (Application) PCR Amplification
Includes Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
Polymerase Phusion High-Fidelity DNA Polymerase
Format Kit
Product Type High-Fidelity PCR Kit
Size (Final Product) 20 kb
Reaction Format Separate Components
Reaction Speed Fast
Hot Start No
Starting Material DNA
Product Line Phusion
9

Thermo Scientific™ Phusion U Green Multiplex PCR Master Mix

High performance multiplex PCR master mix for fast and reliable simultaneous amplification of multiple targets

GC-Rich PCR Performance High
Content And Storage Phusion U Green Multiplex PCR Master Mix includes:
• Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 µM of each dATP, dTTP, dCTP and dGTP
The master mix is supplied with nuclease-free water.
Store at -20°C.
Fidelity (vs. Taq) 25 X
Overhang Blunt
Concentration 2X
Polymerase Phusion U Hot Start DNA Polymerase
Format Tube
Product Type Multiplex PCR Master Mix
Size (Final Product) 2.5 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
10

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with GC Buffer

The Thermo Scientific™ Phusion™ High-Fidelity PCR Master Mixes are high performance enzymes now available in convenient master mix format.

GC-Rich PCR Performance High
Fidelity (vs. Taq) 25 X
Purity DHPLC
Overhang Blunt
Concentration 2X
For Use With (Application) PCR Amplification
Includes With GC Buffer
Polymerase Phusion High-Fidelity DNA Polymerase
Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Size (Final Product) 20 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start No
Starting Material DNA
Product Line Phusion
11

Thermo Scientific™ Phusion™ Plus PCR Master Mix

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Overhang Blunt
Amplicon Size 10 kb genomic DNA, 20 kb low complexity DNA
Storage Requirements Store at -25°C to -15°C
Concentration 2X
For Use With (Application) High Fidelity PCR
Polymerase Phusion Plus DNA Polymerase
Format 2X Master Mix
Size (Final Product) 20 kb or Less
Reaction Format Ready to use master mix
Reaction Speed 15 to 30 s/kb
Hot Start Built-In Hot Start
Starting Material DNA
12

Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/µL)

The most accurate thermostable DNA Polymerase available offering superior performance for cloning and other applications requiring high fidelity.

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2

Both Phusion Green HF and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.
Store at -20°C.
Fidelity (vs. Taq) 52 X
Overhang Blunt
Concentration 2 U/µL
For Use With (Application) PCR Amplification
Polymerase Phusion High-Fidelity DNA Polymerase
Format Stand-alone enzyme
Product Type High-Fidelity DNA Polymerase
Reaction Format Standalone
Reaction Speed Fast
Hot Start No
Starting Material DNA
Product Line Phusion
13

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Perform short PCR protocols without compromising fidelity or yield with this master mix.

Polymerase Phusion Flash II DNA Polymerase
GC-Rich PCR Performance High
Format SuperMix or Master Mix
Fidelity (vs. Taq) 25 X
Product Type High-Fidelity PCR Master Mix
Size (Final Product) 20 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
Product Line Phusion
14

Thermo Scientific™ Phire Green Reaction Buffer

Buffers for use in PCR with Phire Hot Start II DNA Polymerase

15

Thermo Scientific™ Phusion™ Plus DNA Polymerase

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.

Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Format Stand-alone enzyme
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or Less
Reaction Format Separate components
Reaction Speed 15 to 30 s/kb
Hot Start Built-In Hot Start
Overhang Blunt
Amplicon Size 10 kb genomic DNA, 20 kb low complexity DNA
Storage Requirements Store at -25°C to -15°C
For Use With (Application) High Fidelity PCR
Starting Material DNA
16

Thermo Scientific™ Phusion U Green Hot Start DNA Polymerase

Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
Both Phusion Green HF Buffer and Phusion Green GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Store at -20°C.
Fidelity (vs. Taq) 25 X
Overhang Blunt
Concentration 2 U/µL
Polymerase Phusion U Hot Start DNA Polymerase
Format Stand-alone enzyme
Product Type Hot Start DNA Polymerase
Size (Final Product) 20 kb
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
17

Thermo Scientific™ Phusion™ Plus Green PCR Master Mix

Thermo Scientific Phusion Plus Green PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Overhang Blunt
Amplicon Size 10 kb genomic DNA, 20 kb low complexity DNA
Storage Requirements Store at -25°C to -15°C
Concentration 2X
For Use With (Application) High Fidelity PCR
Polymerase Phusion Plus DNA Polymerase
Format 2X Master Mix
Size (Final Product) 20 kb or Less
Reaction Format Ready to use master mix
Reaction Speed 15 to 30 s/kb
Hot Start Built-In Hot Start
Starting Material DNA
18

Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP

GC-Rich PCR Performance Medium
Content And Storage Phusion U Hot Start PCR Master Mix includes:
• Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 µM of each dATP, dTTP, dCTP and dGTP
The master mix is supplied with nuclease-free water.
Store at -20°C.
Fidelity (vs. Taq) 25 X
Overhang Blunt
Concentration 2X
Polymerase Phusion U Hot Start DNA Polymerase
Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Size (Final Product) 20 kb
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
19

Thermo Scientific™ Phusion U Hot Start DNA Polymerase

Thermo Scientific Phusion U DNA Polymerase is a proofreading enzyme (25x Taq) with a proprietary mutation to read through uracils and incorporate dUTP

GC-Rich PCR Performance High
Content And Storage Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

Store at -20°C.
Fidelity (vs. Taq) 25 X
Overhang Blunt
Concentration 2 U/µL
Polymerase Phusion U Hot Start DNA Polymerase
Format Stand-alone enzyme
Product Type Hot Start DNA Polymerase
Size (Final Product) 20 kb
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
20

Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mix

The most accurate hot start DNA polymerase on the market featuring extreme specificity and improved performance.

GC-Rich PCR Performance Medium
Content And Storage Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.
Fidelity (vs. Taq) 52 X
Overhang Blunt
Concentration 2X
For Use With (Application) PCR Amplification
Includes 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO
Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase
Format SuperMix or Master Mix
Product Type Hot Start High-Fidelity PCR Master Mix
Size (Final Product) 20 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Phusion
21

Invitrogen™ Platinum™ Taq DNA Polymerase High Fidelity  DFS Item

Ideal for amplification of DNA fragments when high yields and robust amplification are required

Shipping Condition Dry Ice
Polymerase Platinum™ Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Format Stand-alone enzyme
Fidelity (vs. Taq) 6 X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
Exonuclease Activity 3' - 5', 5' - 3'
Product Line Platinum™
Detection Method Primer-Probe
22

Invitrogen™ SuperFi™ Buffer  DFS Item

Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps

23

Invitrogen™ Platinum™ SuperFi™ DNA Polymerase  DFS Item

Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps

Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300 X
Reaction Format Separate Components
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Product Line Platinum™, SuperFi™
24

Invitrogen™ Platinum™ SuperFi™ PCR Master Mix  DFS Item

Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps

Shipping Condition Wet or Dry Ice
Polymerase Platinum SuperFi DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 300 X
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Product Line Platinum™, SuperFi™
25

Invitrogen™ AccuPrime™ Pfx SuperMix  DFS Item

High-fidelity, high-specificity amplification of DNA fragments

26

Invitrogen™ AccuPrime™ Taq DNA Polymerase, high fidelity  DFS Item

Amplifies nucleic acid templates using antibody-mediated hot-start, a blend of Taq DNA Polymerase and a proofreading enzyme, and AccuPrime accessory proteins for improved PCR fidelity, yield, and specificity over other hot-start DNA polymerases.

Shipping Condition Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 9 X
Exonuclease Activity 3' - 5', 5' - 3'
Detection Method Primer-Probe
Polymerase AccuPrime™ Taq DNA Polymerase High Fidelity
Format Stand-alone enzyme
Product Type DNA Polymerase
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
Product Line AccuPrime™
27

Invitrogen™ SuperFi™ Green Buffer  DFS Item

Amplify even difficult and GC-rich amplicons with superior accuracy (>100X Taq) and minimaloptimization steps

28

Invitrogen™ AccuPrime™ Pfx DNA Polymerase  DFS Item

Provides higher fidelity than Pfu DNA polymerase

Shipping Condition Approved for shipment on Wet or Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 26 X
Overhang Blunt
Exonuclease Activity 3' - 5'
Detection Method Primer-Probe
Polymerase AccuPrime™ Pfx DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 12 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
Product Line AccuPrime™
29

Invitrogen™ Platinum™ SuperFi II Green PCR Master Mix  DFS Item

Invitrogen™ Platinum™ SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) >300X
Overhang Blunt
Concentration 2X
Polymerase Platinum SuperFi II DNA Polymerase
Format SuperMix or Master Mix
Product Type PCR Master Mix
Size (Final Product) 20 kb or Less
Reaction Format SuperMix or Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
Product Line Platinum™, SuperFi™
30

Invitrogen™ AccuPrime™ Taq DNA Polymerase System  DFS Item

Suitable for wide variety of applications, including multiplex PCR, TOPO TA Cloning™, and allele-specific amplifications

Shipping Condition Approved for shipment on Wet or Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 2 X
Overhang 3'-A
Concentration 10X
For Use With (Application) PCR Amplification
PCR Method Multiplex PCR, RT-PCR
Exonuclease Activity 5' - 3'
Detection Method Primer-Probe
Polymerase AccuPrime™ Taq DNA Polymerase
Format Stand-alone enzyme
Product Type DNA Polymerase System
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
Product Line AccuPrime™