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High Fidelity PCR Reagents and Kits

PCR products designed to produce large yields of accurately-replicated target DNA by utilizing a DNA polymerase with a low error rate. Includes master mixes, DNA polymerases, kits, and reagents.
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1 – 15 of 23 results
1

Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
Polymerase Phusion High-Fidelity DNA Polymerase
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2X
For Use With (Application) Standard PCR,High-fidelity PCR
2

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type High-Fidelity DNA Polymerase
Reaction Speed Fast
Hot Start No
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Standard PCR,High-fidelity PCR
3

Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity  DFS Item

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Shipping Condition Dry Ice
Polymerase AccuPrime Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 9X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
For Use With (Application) Hot-start PCR,High-fidelity PCR
Detection Method Primer-probe
4

Invitrogen™ Platinum™ Taq DNA Polymerase, High Fidelity  DFS Item

Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.

Shipping Condition Dry Ice
Polymerase Platinum Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 6X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Reaction Speed Standard
Hot Start Built-In Hot Start
For Use With (Application) Hot-start PCR,High-fidelity PCR
Detection Method Primer-probe
5

Invitrogen™ Platinum™ PCR SuperMix High Fidelity  DFS Item

Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.

Shipping Condition Wet or Dry Ice
Polymerase Platinum Taq DNA Polymerase High Fidelity
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 6X
Size (Final Product) 15 kb or less
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start Built-In Hot Start
Overhang Mixed
Concentration 1.1X
For Use With (Application) Hot-start PCR,High-fidelity PCR
6

Thermo Scientific™ Phusion™ Plus DNA Polymerase

Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.

Shipping Condition Room Temp or Wet Ice
Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or less
Reaction Format Separate components
Reaction Speed 15 to 30 sec./kb
Hot Start Built-In Hot Start
Overhang Blunt
For Use With (Application) Hot-start PCR,High-fidelity PCR
7

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

Shipping Condition Dry Ice
Polymerase Phusion Flash II DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type High-Fidelity PCR Master Mix
Reaction Speed Fast
Color Colorless
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
8

Thermo Scientific™ Phusion High-Fidelity PCR Kit

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
Polymerase Phusion High-Fidelity DNA Polymerase
GC-Rich PCR Performance High
Content And Storage • Phusion DNA Polymerase (2 U/μL)
• 5X Phusion GC & HF Buffer
• dNTP mix (10 mM each)
• Control template (λ DNA) and DNA size standard
• 1.3 kb & 10 kb primers (4 μM each)
• 50 mM MgCl2 solution and DMSO

Store at –20°C.
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Separate Components
Product Type High-Fidelity PCR Kit
Reaction Speed Fast
Hot Start No
Overhang Blunt
For Use With (Application) Standard PCR,High-fidelity PCR
9

Thermo Scientific™ Phusion U Hot Start DNA Polymerases

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Product Type Hot Start DNA Polymerase
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Hot-start PCR,High-fidelity PCR
10

Thermo Scientific™ Phusion U Multiplex PCR Master Mixes

Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.

Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
Fidelity (vs. Taq) 25X
Size (Final Product) 2.5 kb or less
Reaction Format SuperMix or Master Mix
Product Type Multiplex PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
11

Thermo Scientific™ Phusion Hot Start II High-Fidelity PCR Master Mixes

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
Polymerase Phusion Hot Start II High-Fidelity DNA Polymerase
GC-Rich PCR Performance Medium
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start High-Fidelity PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
12

Thermo Scientific™ Phusion™ Plus PCR Master Mixes

Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.

Shipping Condition Dry Ice
Polymerase Phusion Plus DNA Polymerase
GC-Rich PCR Performance High
Fidelity (vs. Taq) >100X
Size (Final Product) 20 kb or less
Reaction Format Ready to use master mix
Reaction Speed 15 to 30 sec./kb
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR,High-fidelity PCR
13

Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 52X
Size (Final Product) 20 kb or less
Reaction Format Standalone
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
For Use With (Application) Hot-start PCR,High-fidelity PCR
14

Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Shipping Condition Dry Ice
Polymerase Phusion U Hot Start DNA Polymerase
GC-Rich PCR Performance Medium
Content And Storage • Phusion U Hot Start DNA Polymerase
• 2X reaction buffer
• 3 mM MgCl2
• 400 μM each of dATP, dTTP, dCTP and dGTP

Master mix is supplied with nuclease-free water.
Store at -20°C.
Fidelity (vs. Taq) 25X
Size (Final Product) 20 kb or less
Reaction Format SuperMix or Master Mix
Product Type Hot Start PCR Master Mix
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
For Use With (Application) Hot-start PCR
15

Thermo Scientific™ Phusion Site-Directed Mutagenesis Kit

Generate mutations with this fast and reliable system.