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Invitrogen™ SuperScript™ IV VILO™ Master Mix DFS Item

SuperScript™ IV VILO™ Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.

$1,032.00 - $7,690.00

Specifications

Concentration 5X
Detection Method qPCR
Final Product Type First-Strand cDNA
Format Master Mix
GC-Rich PCR Performance High
View More Specs

Products 2
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
11756050 DFS Item
View Documents Promotion Details
Invitrogen™
11756050
50 Reactions
Each for $1,032.00
 
11756500 DFS Item
View Documents Promotion Details
Invitrogen™
11756500
500 Reactions
Each for $7,690.00
 
Description

Description

SuperScript™ IV VILO™ (SSIV VILO) Master Mix is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications. Inclusion of ezDNase, further accelerates the RT-qPCR workflow through an extremely simplified genomic DNA removal step. This master mix is also available without ezDNase, which can be purchased separately.

Features of SuperScript IV VILO Master Mix include:
  • Super-fast RT reaction in just 10 minutes and gDNA removal in 2 minutes
  • Super-high yields—lower Ct values by more than 2 cycles ahead of all other reverse transcription reagents
  • Super-convenient one-tube cDNA reaction master mix for two-step RT-qPCR
  • Super-efficient reaction even with low template amounts and suboptimal purity samples
SSIV VILO master mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript™ IV Reverse Transcriptase and further optimized buffer. These components enable efficient cDNA synthesis at higher temperatures and in less time. SSIV VILO master mix provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates. It is your new tool for more efficient and reproducible RT-qPCR.

For even better performance, some SuperScript IV VILO Master Mix products include ezDNase (also available as a separate purchase) for genomic DNA removal to further accelerate the RT-qPCR workflow. The extremely simplified genomic DNA removal step dramatically reduces the time of the entire reverse transcription protocol and reduces possible variation in gene expression due to RNA loss or damage during conventional DNase treatment.

SSIV VILO master mix gives you more cDNA in less time with less pipeting, less variation, less reaction inhibition, less gDNA interference, and with less sample than all the other cDNA synthesis kits or master mixes for RT-qPCR.

Source
Purified from E. coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance, and to reduce RNase H activity.

Performance and quality testing
Assayed for endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease, as well as yield and length of cDNA product.

For Research Use Only. Not for use in diagnostic procedures.
Specifications

Specifications

5X
First-Strand cDNA
High
Master Mix
Reverse Transcription
RNA
50°C
Reduced
RT-qPCR
qPCR
Master Mix
RT-qPCR
10 min.
Up to 10 kb
Reverse Transcription
SuperScript IV
Dry Ice
SDS
Product Certifications