Hot Start PCR Reagents and Kits
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Invitrogen™ PCR Master Mix Starter Pack
The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research.
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
| Polymérase | Phire Hot Start II DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Fast |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Matériau de départ | DNA |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| Polymérase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Taille (produit final) | 5 kb or less |
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
| Polymérase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
| Polymérase | Phire Hot Start II DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Hot Start PCR Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Concentration | 2X |
| Matériau de départ | DNA |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| GC-Rich PCR Performance | Low |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Type de produit | DNA Polymerase With Buffer I |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
| Polymérase | AccuPrime GC-Rich DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Taille (produit final) | 5 kb or less |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | High |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| Taille (produit final) | 5 kb or less |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | Medium |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format | Tube |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Hot Start PCR Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 2X |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
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AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Sustainable packaging |
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | PCR Master Mix |
| En surplomb | 3'-A |
| Concentration | 2X |
| Conditions d’expédition | Room Temp or Wet Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
| Polymérase | AmpliTaq Gold 360 DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Activité exonucléasique | 5' - 3' |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ True Allele™ PCR Premix
Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
| Polymérase | Platinum II Taq Hot-Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Fast or Standard |
| Format de réaction | Separate Components |
| Type de produit | Hot Start DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 2X |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ Platinum™ Multiplex PCR Master Mix
The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.
| Capacité multiplex | Up to 20-plex |
|---|---|
| Polymérase | Platinum Taq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| Taille (produit final) | 2.5 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR,Multiplex PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase, LD (Low DNA) with Gold Buffer and MgCl2
Applied Biosystems AmpliTaq Gold DNA Polymerase, LD (Low DNA) is a highly purified version of AmpliTaq Gold DNA Polymerase that significantly reduces contaminating bacterial DNA sequences commonly present in recombinant protein preparations.
| GC-Rich PCR Performance | Low |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase |
| Pureté ou qualité | LD (Low DNA) |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
| Polymérase | AmpliTaq Gold DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Activité exonucléasique | 5' - 3' |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Applied Biosystems™ AmpliTaq Gold™ Fast PCR Master Mix
The Applied Biosystems AmpliTaq Gold Fast PCR Master Mix is a premix, hot start chemistry for end-point PCR analysis.
| GC-Rich PCR Performance | High |
|---|---|
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Fast PCR Master Mix |
| En surplomb | 3'-A |
| Concentration | 2X |
| Conditions d’expédition | Wet Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
| Polymérase | AmpliTaq Gold DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
| Taille (produit final) | 1.5 kb or less |