Hot Start PCR Reagents and Kits
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Invitrogen™ PCR Master Mix Starter Pack
The PCR Master Mix Starter Pack is composed of three different PCR master mixes for easy evaluation of their suitability for use in your research.
Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
| Polymérase | Phire Hot Start II DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Fast |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Matériau de départ | DNA |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| Polymérase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Type de produit | DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Taille (produit final) | 5 kb or less |
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
| Polymérase | DreamTaq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Vitesse de réaction | Standard |
| Format de réaction | SuperMix or Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
| Polymérase | Phire Hot Start II DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Hot Start PCR Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Concentration | 2X |
| Matériau de départ | DNA |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Cloning,Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| GC-Rich PCR Performance | Low |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Activité exonucléasique | 5' - 3' |
| Type de produit | DNA Polymerase With Buffer I |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase
Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.
Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.
| Polymérase | Tsp Genotyping Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Taille (produit final) | 500 bp or less |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ Platinum™ Hot Start PCR Master Mixes (2X)
Invitrogen Platinum Hot Start PCR Master Mix offers Platinum Taq DNA polymerase in an optimized PCR buffer with magnesium and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | Medium |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Standard |
| Format | Tube |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Hot Start PCR Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 2X |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
| Polymérase | AccuPrime GC-Rich DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 2X |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Taille (produit final) | 5 kb or less |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
Invitrogen™ AccuPrime™ Taq DNA Polymerase System
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
| GC-Rich PCR Performance | Low |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | DNA Polymerase System |
| En surplomb | 3'-A |
| Méthode PCR | 1-step RT-qPCR,Multiplex qPCR |
| Concentration | 10X |
| Conditions d’expédition | Wet or Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
| Polymérase | AccuPrime Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 2X |
| Hot Start | Built-In Hot Start |
| Taille (produit final) | 5 kb or less |
Invitrogen™ Platinum™ Direct PCR Universal Master Mix
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Invitrogen Platinum Direct PCR Universal Master Mix is designed to amplify DNA directly from various samples without the need to purify DNA.
| Polymérase | Platinum II Taq Hot-Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Concept écologique | Less hazardous, less waste |
| Fidélité (par rapport à Taq) | 1X |
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Direct PCR Universal Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | 3'-A |
| Concentration | 2X |
| Taille (produit final) | 8 kb |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR,Real Time PCR (qPCR) |
Applied Biosystems™ True Allele™ PCR Premix
Applied Biosystems True Allele PCR Premix is a PCR master mix that contains AmpliTaq Gold DNA Polymerase, dNTPs, MgCl2, and buffer.
Invitrogen™ Platinum™ Taq DNA Polymerase, DNA-free
Invitrogen Platinum Taq DNA Polymerase, DNA-free, is manufactured using a novel, closed single-use system together with stringent quality-control testing to minimize the risk of contamination with bacterial and human DNA.
| GC-Rich PCR Performance | Low |
|---|---|
| Vitesse de réaction | Standard |
| Format de réaction | Separate Components |
| Type de produit | Taq DNA Polymerase |
| En surplomb | 3'-A |
| Concentration | 5 U/μL |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
| Polymérase | Platinum Taq DNA Polymerase |
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Hot Start | Built-In Hot Start |
| Matériau de départ | DNA |
| Taille (produit final) | 5 kb or less |
Invitrogen™ Platinum™ II Hot-Start PCR Master Mixes (2X)
Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup.
| GC-Rich PCR Performance | High |
|---|---|
| Fidélité (par rapport à Taq) | 1X |
| Format | Tube |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| Taille (produit final) | 5 kb or less |
| À utiliser avec (application) | Hot-start PCR |