Reverse Transcription Products
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Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Invitrogen™ SuperScript™ IV First-Strand Synthesis System
The SuperScript IV First-Strand Synthesis System for RT-PCR, available with or without ezDNase, is optimized for synthesis of first-strand cDNA from purified poly(A)+ or total RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Format | Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 10 min. |
| Reagent Type | Reverse Transcription |
| Quantity | Each |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ II Reverse Transcriptase
Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT.
| Shipping Condition | Wet Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript II |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR).
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Format | Super Mix |
| Reverse Transcriptase | SuperScript III |
| Reaction Format | Master Mix |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR) |
| Detection Method | qPCR |
Invitrogen™ SuperScript™ III Reverse Transcriptase
Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 12.3 kb |
| Reaction Format | Separate components |
| Reaction Speed | 30 to 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 50°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
| GC-Rich PCR Performance | High |
|---|---|
| Format | 1-step RT-PCR Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Master Mix |
| Reagent Type | Reverse Transcription |
| Hot Start | Built-in Hot Start |
| Optimal Reaction Temperature | 50°C to 65°C |
| Technique | 1-step RT-PCR |
| Starting Material | RNA |
| PCR Method | 1-step RT-PCR |
| Multiplex Capability | Yes |
| Detection Method | Gel Electrophoresis |
Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Applied Biosystems™ TaqMan™ Reverse Transcription Reagents
TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.
| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 5 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Concentration | 50 U/μL |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR) |
Invitrogen™ MultiScribe™ Reverse Transcriptase
MultiScribe Reverse Transcriptase is a recombinant moloney murine leukemia virus (rMoMuLV) reverse transcriptase optimized for TaqMan-based assays.
Thermo Scientific™ Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity.
| Concentration | 200 U/μL |
|---|
Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid H Minus Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid H Minus |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Optimal Reaction Temperature | 42°C to 45°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
| Shipping Condition | Dry Ice |
|---|---|
| Content And Storage | • RevertAid Reverse Transcriptase (200 U/μL) |
| Reverse Transcriptase | RevertAid |
| Final Product Type | First-Strand cDNA |
| Concentration | 200 U/μL |
| Format | Stand-alone Enzyme |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Separate components |
| Reaction Speed | 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 42°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
| Shipping Condition | Wet Ice |
|---|---|
| Format | Stand-alone Enzyme |
| Reverse Transcriptase | M-MLV |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Separate components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 50 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Concentration | 200 U/μL |
| Starting Material | RNA |
Thermo Scientific™ RevertAid RT Reverse Transcription Kit
The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.