Reverse Transcription Products
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Invitrogen™ Second Strand cDNA Synthesis Kit
Invitrogen™ Second Strand cDNA Synthesis Kit is design to produce double-stranded cDNA using first strand reaction without the need for intermediate organic extraction or ethanol precipitation steps. This convenient single-tube format speeds up the synthesis procedure and maximizes cDNA recovery. The kit contains premixed components to reduce the number of pipetting steps necessary to complete the procedure.
| Format de réaction | Separate Components |
|---|---|
| Type de produit | cDNA Synthesis Kit |
| Type de produit final | Double Stranded cDNA |
| À utiliser avec (application) | Cloning |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Gel Electrophoresis |
| Vitesse de réaction | 30 min. |
| Format de réaction | Premixed Components |
| Type de produit final | PCR Amplified cDNA |
| Activité de la ribonucléase H | Reduced |
| Méthode PCR | 1-step RT-PCR |
| Conditions d’expédition | Dry Ice |
| Polymérase | Platinum Taq High Fidelity |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | One-Step RT-PCR System |
| Hot Start | Built-In Hot Start |
| Technique | 1-Step RT-PCR |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A)+ or total RNA. RNA targets from 100 bp to >12 kb can be detected with this system.
Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit
The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.
| GC-Rich PCR Performance | High |
|---|---|
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 50 min. |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Technique | Reverse Transcription |
| Matériau de départ | Cells |
| Taille (produit final) | Up to 4.5 kb |
| Transcriptase inverse | SuperScript III |
| À utiliser avec (application) | Real Time PCR (qPCR) |
Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
| Température de réaction optimale | 37°C |
|---|---|
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 30 to 60 min. |
| Format | Kit |
| Format de réaction | Premixed Components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 7 kb |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | MultiScribe |
| À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
Invitrogen™ SuperScript™ IV VILO™ Master Mix
SuperScript IV VILO (SSIV VILO) Master Mix, available with or without ezDNase, is a reaction master mix designed for fast, sensitive, and reproducible cDNA synthesis in RT-qPCR applications.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | 10 min. |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Concentration | 5X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | Master Mix |
| Quantité | Each |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript IV |
Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents
The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.
Invitrogen™ SuperScript™ IV Single Cell/Low Input cDNA PreAmp Kit
The Invitrogen SuperScript IV Single Cell/Low Input cDNA PreAmp Kit is designed for efficient cDNA synthesis and amplification directly from intact single cells (1–1,000) or low amounts of total RNA (2 pg–10 ng).
| Polymérase | Platinum SuperFi U DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 30 min. |
| Format | Kit |
| Type de produit final | PCR Amplified cDNA |
| Technique | Reverse Transcription, Preamplification |
| Matériau de départ | Cells or RNA |
| Taille (produit final) | Up to 10 kb |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | SuperScript IV |
| À utiliser avec (application) | Next-Generation Sequencing (NGS),Real Time PCR (qPCR) |
Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit
The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.
| Format de réaction | Separate Components |
|---|---|
| Type de produit | cDNA Synthesis Kit |
| Gamme de produits | Maxima |
| Type d’échantillon | RNA |
| Type de produit final | Double Stranded cDNA |
| Transcriptase inverse | Maxima H Minus |
| À utiliser avec (application) | Cloning |
Invitrogen™ M-MLV Reverse Transcriptase (200 U/μL)
M-MLV Reverse Transcriptase is a recombinant DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid.
| Température de réaction optimale | 37°C |
|---|---|
| Type de réactif | Reverse Transcription |
| Vitesse de réaction | 50 min. |
| Format | Stand-alone Enzyme |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Concentration | 200 U/μL |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 7 kb |
| Conditions d’expédition | Wet Ice |
| Transcriptase inverse | M-MLV |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Gel Electrophoresis |
| Vitesse de réaction | 30 min. |
| Format de réaction | Premixed Components |
| Type de produit final | PCR Amplified cDNA |
| Activité de la ribonucléase H | Reduced |
| Méthode PCR | 1-step RT-PCR |
| Conditions d’expédition | Dry Ice |
| Polymérase | Platinum Taq DNA Polymerase |
| Température de réaction optimale | 50°C |
| Type de réactif | Reverse Transcription |
| Format | One-Step RT-PCR System |
| Hot Start | Built-In Hot Start |
| Technique | 1-Step RT-PCR |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 4.5 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit
The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | qPCR |
| Vitesse de réaction | 60 min. |
| Format de réaction | Master Mix |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Reduced |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Kit |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 10 kb |
| Transcriptase inverse | SuperScript III |
Invitrogen™ SuperScript™ IV One-Step RT-PCR System
The Invitrogen SuperScript One-Step RT-PCR System, available with or without ezDNase, combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Gel Electrophoresis |
| Vitesse de réaction | 10 min. |
| Format de réaction | Master Mix |
| Type de produit final | PCR Amplified cDNA |
| Activité de la ribonucléase H | Reduced |
| Méthode PCR | 1-step RT-PCR |
| Conditions d’expédition | Dry Ice |
| Polymérase | Platinum SuperFi |
| Température de réaction optimale | 45°C to 60°C |
| Type de réactif | Reverse Transcription |
| Format | Kit |
| Hot Start | Built-In Hot Start |
| Quantité | 1 Ea. |
| Technique | 1-step RT-PCR |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 14 kb |
| Transcriptase inverse | SuperScript IV |
Applied Biosystems™ High-Capacity cDNA Reverse Transcription Kit
The High Capacity cDNA Reverse Transcription Kit, available with or without RNase Inhibitor, contains all components necessary for the quantitative conversion of up to 2 μg of total RNA into single-stranded cDNA in a single 20 μL reaction.
| Type de réactif | Reverse Transcription |
|---|---|
| Vitesse de réaction | 120 min. |
| Format | Kit |
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Quantité | 1 Ea. |
| Activité de la ribonucléase H | Yes |
| Technique | Reverse Transcription |
| Concentration | 50 U/μL |
| Matériau de départ | RNA |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | MultiScribe |
Thermo Scientific™ RevertAid Reverse Transcriptase (200 U/μL)
Thermo Scientific RevertAid Reverse Transcriptase (RT) is a recombinant M-MuLV RT. It differs from the M-MuLV RT by its structure and catalytic properties.
| Vitesse de réaction | 60 min. |
|---|---|
| Format de réaction | Separate components |
| Type de produit final | First-Strand cDNA |
| Activité de la ribonucléase H | Yes |
| Concentration | 200 U/μL |
| Conditions d’expédition | Dry Ice |
| Contenu et stockage | • RevertAid Reverse Transcriptase (200 U/μL) |
| Température de réaction optimale | 42°C |
| Type de réactif | Reverse Transcription |
| Format | Stand-alone Enzyme |
| Technique | Reverse Transcription |
| Matériau de départ | RNA |
| Taille (produit final) | Up to 13 kb |
| Transcriptase inverse | RevertAid |