missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Reverse Transcription Products
  6. cDNA Synthesis Kits and Master Mixes
  7. Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit

Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit

Catalog No. 11917020
Encompass
Change view
Click to view available options
Quantity:
10 Reactions
50 Reactions
2 product options available for selection
Product selection table with 2 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
11917020 50 Reactions
11917010 10 Reactions
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 2 options available.
2 options
Catalog No. 11917020 Supplier Invitrogen™ Supplier No. 11917020

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.

Description

Includes

Reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50μg total RNA or 0.2-5μg mRNA into double-stranded cDNA.

Requires

Primer

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA).

Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.

  • Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
  • Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
  • Optimal temperature: 42°C

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription

Order Info

Shipping Condition: Wet Ice

Specifications

Content And Storage The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 μg total RNA or 0.2-5 μg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored.
Format Kit
Labeling Method Direct Labeling
Optimal Reaction Temperature 42°C
Product Line SuperScript
Product Type cDNA Synthesis Kit
Quantity 50 Reactions
Reverse Transcriptase SuperScript™ II
Shipping Condition Wet Ice
For Use With (Application) Microarray Analysis
Final Product Type cDNA (Double-Stranded)
Sample Type RNA
Show More Show Less

Frequently Asked Questions (FAQs)

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

I'd like to generate double-stranded DNA (dsDNA) from mRNA. Do you have a kit for this?

To generate double-stranded cDNA, we recommend the Maxima H Minus Double-Stranded cDNA Synthesis Kit (Cat. No. K2561) or Second Strand cDNA Synthesis Kit (Cat. No. A48570).

For Research Use Only. Not for use in diagnostic procedures.