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Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit DFS Item

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Manufacturer:  Invitrogen™ LS11917020

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Catalog No. 11917020

Please call Customer Service at 1-800-234-7437 or send an email to help@thermofisher.com for assistance.


Requires : Primer

Description

Description

Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA).

Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.

  • Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
  • Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
  • Optimal temperature: 42°C

DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription

Specifications

Specifications

The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 µg total RNA or 0.2-5 µg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored.
Direct Labeling
SuperScript™
SuperScript™ II
Wet Ice
50 reactions
cDNA (Double-Stranded)
42° C
Separate Components
RNA
Microarray Analysis
SDS
Documents

For Research Use Only. Not for use in diagnostic procedures.