Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit
Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis
Manufacturer: Invitrogen™ LS11917020
Contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA).
Simple, reliable, and trouble-free
The SuperScript Double-Stranded cDNA Synthesis Kit offers a simple, reliable, and trouble-free method by which RNA templates can be converted to cDNA for use in cloning and library construction experiments. The reactions described in the SuperScript Double-Stranded cDNA Synthesis Kit protocol are designed to convert 25–50μg total RNA, or 0.2–5μg mRNA, into first- and second-strand cDNA (supply your own primer). Enjoy the benefits of a tested protocol and reagents of consistent quality. To get the best possible cDNA product using this kit, use a high-quality RNA substrate.
- Reliability: each buffer, reagent, and enzyme in the kit is of the same high quality you expect from Life Technologies
- Performance: SuperScript II is an enzyme you can trust for high-yield, high-quality, general-purpose, first-strand synthesis
- Optimal temperature: 42°C
DNA and RNA Purification and Analysis, DNA Labeling, Expression Array Labeling, Gene Expression Analysis and Genotyping, Microarray Analysis, Nucleic Acid Labeling and Oligo Synthesis, PCR and Real-Time PCR, Reverse Transcription
|The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 µg total RNA or 0.2-5 µg mRNA into double-stranded cDNA. All components are guaranteed stable for 6 months when properly stored.|
For Research Use Only. Not for use in diagnostic procedures.