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Substrates

Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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1

Thermo Scientific™ beta-Galactosidase Assay Reagent

Lyse cultured mammalian cells and measure beta-galactosidase activity using compatible M-PER Cell Lysis Reagent and colorimetric b-Gal Assay Reagent.

Substrate Beta-Gal Substrate
Assay Reporter Enzyme
Substrate Properties Chemical Substrate
Content And Storage Store at -20°C.
Format 96-well Plate
Product Type Pierce beta-Galactosidase Assay Reagent
For Use With (Equipment) Microplate Reader
Form Liquid
Label or Dye Enzyme Labeled
Target Enzyme Beta-Galactosidase
For Use With (Application) Absorbance
Product Line Pierce
Detection Method Colorimetric
2

Thermo Scientific™ PI Lipid Kinase Substrate  DFS Item

Specifically optimized for use with the Class II (PIK3C2A & PIK3C2B) PI3 kinases.

3

Thermo Scientific™ Vivid™ EOMCC Substrate  DFS Item

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs.

Shipping Condition Approved for shipment on Wet or Dry Ice
Substrate EOMCC
Substrate Properties Chemical Substrate
Content And Storage Vivid™ CYP450 Screening kits include:
• Vivid™ CYP450 Fluorogenic substrates (also available separately)
• BACULOSOMES™ CYP450 reagents
• Fluorescent standards
• NADPH-regeneration system

Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Substrate Type Cytochrome P450 Substrate
Product Line VIVID
Detection Method Fluorescence
5

Thermo Scientific™ ZIPtide Substrate  DFS Item

A peptide substrate for the Ca/Calmodulin-dependent protein kinase (CaM) family including CAMK1 and DAPK1.

6

Invitrogen™ MUP (4-Methylumbelliferyl Phosphate, Free Acid)  DFS Item

MUP is a fluorescent substrate for alkaline phosphatases

11

Invitrogen™ DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)  DFS Item

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).

13

Thermo Scientific™ CDK7/9tide Substrate  DFS Item

A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9).

15

Thermo Scientific™ GFP-4E-BP1  DFS Item

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).