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Substrates

Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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3

CCF2-FA (free acid)  DFS Item

CCF2-FA (free acid)

Shipping Condition Approved for shipment on Wet or Dry Ice
Substrate CCF2-FA
Cell Permeability Cell-Impermeant
Substrate Properties Chemical Substrate
Content And Storage Store in freezer (-5 to -30°C).
Substrate Type Beta-Lactamase Substrate
Product Line GeneBLAzer
Detection Method Fluorescence
4

Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)  DFS Item

PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.

5

Thermo Scientific™ Fluorescein-PKC Substrate  DFS Item

A fluorescein-labeled peptide substrate for the Protein Kinase C (PKC) family.

7

Thermo Scientific™ LanthaScreen™ Tb-DUB Substrate  DFS Item

Deubiquitinating enzymes (DUBs) play housekeeping functions by maintaining pools of active ubiquitin, and regulatory function by rescuing specific target proteins from degradation by the proteasome.

8

Invitrogen™ Alexa Fluor™ 647-Poly-GT Peptide Substrate  DFS Item

A general AlexaFluor647-labeled peptide substrate consisting of a polymer of glutamate, and tyrosine that is used to monitor tyrosine kinase acitivty.

Shipping Condition Dry Ice
Excitation/Emission 340/665 nm
Substrate Random (Glu:Tyr) polymer in a ratio of 4:1
Substrate Properties Peptide-Based Substrate
Form Liquid
Label or Dye Alexa Fluor™ 647
Color Far-Red
Target Enzyme Tyrosine Kinases
Substrate Type Kinase Substrate
Validated Kinases EGFR
Technique TR-FRET
Detection Method Fluorescence
10

Invitrogen™ DQ™ Green BSA - Special Packaging  DFS Item

Hydrolysis of substrate yields brightly fluorescent product

11

Invitrogen™ DiFMUP (6,8-Difluoro-4-Methylumbelliferyl Phosphate)  DFS Item

Continuously assay acid phosphatases at low pH

Shipping Condition Room Temperature
Excitation/Emission 358⁄455
Substrate Phosphatase Substrate
Substrate Properties Chemical Substrate
Content And Storage Store in freezer (-5 to -30°C).
For Use With (Equipment) Microplate Reader
Form Powder
Label or Dye DiFMU
Color Blue
Target Enzyme Phosphatases
Detection Method Fluorescence
12

Invitrogen™ DDAO galactoside (9H-(1,3-Dichloro-9,9-Dimethylacridin-2-One-7-yl) β-D-Galactopyranoside)  DFS Item

The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).