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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (671)
Protease Inhibitors and Phosphatase Inhibitors (280)
Magnetic Affinity Separation Particles (123)
Gel Filtration Media and Columns (85)
Spin Columns and Gravity-flow Columns (53)
Cell Lysis Detergents (49)
In vitro Protein Expression Systems (46)
Miscellaneous Immobilized Affinity Ligands (45)
Biotin-Binding Affinity Resins and Beads (34)
Miscellaneous Immobilization Supports (28)
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Cell Lysis Enzymes (23)

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1

Gibco™ ExpiSf™ Protein Production Kit

Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.

Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Expression Mechanism Cell-Based Expression
Format Liquid
Compatible Cells Insect Cells
Classification Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Expression System Baculovirus
Product Line ExpiSf
2

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce
Detection Method Colorimetric
3

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Particle Size 1 μm
Ligand Type Streptavidin
Type Magnetic Affinity Bead
Target Biotin
Content And Storage Upon receipt store at 4°C. Product is shipped on an ice pack.
Protein Form Recombinant
Form Liquid
Concentration Slurry: 10 mg/mL, 1% solids
Product Line Pierce
4

Invitrogen™ DynaMag™-2 Magnet  DFS Item

The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.

5

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
6

MilliporeSigma™ Calbiochem™ Cathepsin G Inhibitor I

The Cathepsin G Inhibitor I, also referenced under CAS 429676-93-7, controls the biological activity of Cathepsin G. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

Content And Storage Protect from light
CAS 429676-93-7
Form Solid
Target Proteins cathepsin G
Solubility DMSO
Color White
Purity 98% by HPLC
Molecular Formula C36H33N2O6P
Formula Weight 620.6g/mol
7

MilliporeSigma™ Calbiochem™ Okadaic Acid, Prorocentrum sp.

Okadaic Acid, CAS 78111-17-8, is a highly potent inhibitor of protein phosphatase 1 (IC50 = 10-15 nM) and 2A (IC50 = 0.1 nM).

Content And Storage Protect from light
CAS 78111-17-8
Form Glassy Solid
Target Proteins Protein phosphatase
Solubility MeOH, EtOH, DMF, CHCl3
Color Colorless
Purity 95% by HPLC
Molecular Formula C44H68O13
Formula Weight 805g/mol
8

MilliporeSigma™ Calbiochem™ NAE Inhibitor, MLN4924

NAE Inhibitor, MLN4924, CAS 951950-33-7, is an AMP mimetic that forms a NAE-catalyzed NEDD8 adduct, which acts as a tight-binding, ATP-competitive NAE inhibitor (IC50 = 4.7nM).

9

MilliporeSigma™ Calbiochem™ InSolution™ Y-27632 in DMSO

InSolution™ Y-27632 is a 10mm solution in DMSO. A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140nM for p160ROCK).

10

Thermo Scientific™ Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)

Prevent protein degradation and stabilize phosphorylation with this all-in-one cocktail of protease and phosphatase inhibitors.

Buffer Lysis Buffer
Content And Storage Upon receipt store at 4°C
Inhibitors Protease Inhibitor, Phosphatase Inhibitor
Form Liquid
Product Type Protease and Phosphatase Inhibitor Cocktail
Reagent Type Protease Inhibitor, Phosphatase Inhibitor
Product Line Halt
11

MilliporeSigma™ Calbiochem™ JNK Inhibitor II

JNK Inhibitor II. SP600125, CAS 129-56-6, is a potent, cell-permeable, selective, and ATP competitive inhibitor of c-Jun N-terminal kinase (JNK; IC50 = 40 nM for JNK-1 & JNK-2 & 90 nM for JNK-3).

Content And Storage Protect from light
CAS 129-56-6
Form Solid
Target Proteins JNK 1, JNK 2, JNK 3
Solubility DMSO
Color Yellowish Orange
Purity 98% by HPLC
Molecular Formula C14H8N2O
Formula Weight 220.2g/mol
12

MilliporeSigma™ Calbiochem™ JAK Inhibitor I

JAK Inhibitor I, CAS 457081-03-7, is a potent, reversible, cell-permeable, and ATP-competitive inhibitor of JAK 1 (IC50 = 15 nM), JAK2 (IC50 = 1 nM), JAK3 (Ki = 5 nM) and Tyk2 (IC50 = 1 nM).

Content And Storage Protect from light
CAS 457081-03-7
Form Solid
Target Proteins murine JAK1
Solubility DMSO
Color Off-white
Purity 98% by HPLC
Molecular Formula C18H16FN3O
Formula Weight 309.3g/mol
13

MilliporeSigma™ Calbiochem™ InSolution™ Q-VD-OPh, Non-O-methylated

InSolution™ Q-VD-OPh, Non-O-methylated, is a cell-permeable, irreversible, broad-spectrum inhibitor of caspases (IC50 = 50, 100, 430, and <25 nM for caspase-1,-8, -9, and -3, respectively).

Content And Storage Hygroscopic, Protect from light
Form Liquid
Target Proteins caspase-1
Formulation Supplied as a 10 mM (1mg/195μl) solution in DMSO.
Purity 90% by HPLC
Molecular Formula C26H25F2N3O6
Formula Weight 513.8g/mol
14

MilliporeSigma™ Calbiochem™ Cisplatin

Reported to sensitize glioma cells to TNF-α-induced apoptosis.

15

MilliporeSigma™ Calbiochem™ Conduritol B Epoxide

Conduritol B Epoxide, CAS 6090-95-5, is an irreversible Inhibitor of glucocerebrosidase in neurons. Also inhibits α-glucosidase activity in a variety of species.