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Thermo Scientific™ HisPur™ Ni-NTA Spin Purification Kit, 3 mL
Description
Perform routine affinity purification of His-tagged fusion proteins with Thermo Scientific™ HisPur Ni-NTA Resin, a high-capacity, high-performance nickel-IMAC resin.
HisPur Ni-NTA Resin is available in several package sizes of resin slurry, three sizes of centrifuge-ready columns, complete purification kits, two sizes of FPLC-ready chromatography cartridges, and 96-well filter plates for high through-put needs. With these options, high-yield His-tag protein purification is possible at nearly any scale.
Highlights:
High capacity – bind up to 60mg of 6xHis-tagged protein per milliliter of resin
Versatile – purify proteins using native or denaturing conditions
Compatible – use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Flexible – available in multiple formats including bulk resin, spin columns and chromatography cartridges
Cost-effective – reuse the same batch of resin at least five times
Easy to use – pre-formulated buffers available for kit formats
Available Formats:
- Resin Slurries – crosslinked 6% beaded agarose; 10mL, 100mL, 500mL bottles
- Spin Columns – 0.2mL (microcentrifuge), 1mL, 3mL columns
- Purification Kits – complete kits in all three column sizes
- Chromatography Cartridges – compatible with automated liquid chromatography systems and with syringe processing
- Bulk and Custom – let us know your needs for bulk HisPur Ni-NTA Resin
Includes:
Kits contain HisPur Ni-NTA Columns
Specifications
Specifications
| Capacity (Metric) | 1 mL/Column |
| Description | HisPur Ni-NTA Spin Purification Kit, 3mL |
| Content And Storage | Sufficient For: Purifying up to 180 mg of His-tagged protein per column • HisPur Ni-NTA Spin Columns, 3 mL, 5 columns • Phosphate-buffered Saline (10X), 30 mL • Imidazole (2M), 25 mL • Column Plugs, 5 plugs Store at 4°C. |
| Product Line | HisPur |
| Quantity | 5 Column Kit |
| Target | His-Tagged Protein |
| Type | Spin Column Kit |
| Stationary Phase | Divalent Nickel, Metal Chelate |
| Sufficient For | 180 mg of His-Tagged Protein per Column |
| Format | Spin Column |
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Frequently Asked Questions (FAQs)
Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.
ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).
Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.
Nickel has a higher affinity for histidines than does cobalt. It binds multiple histidines more tightly than cobalt, and requires more stringent conditions than is necessary for cobalt. The lower affinity of cobalt for multiple histidines typically results in less nonspecific binding of histidine-rich proteins that lack a his-tag compared to nickel resins.
HisPur Ni-NTA resin has a high capacity of up to 60 mg of 6xHis-tagged protein per milliliter. It is a versatile resin that can work under both native and denaturing conditions, and can also be used with a variety of lysis reagents and buffer additives.
HisPur Cobalt resin utilizes proprietary tetradentate chelating resin charged with cobalt. This system recovers highly purified protein with lower imidazole concentrations and has low metal leeching properties.
For Research Use Only. Not for use in diagnostic procedures.