Crosslinking Reagents
Thermo Scientific™ BM(PEG)2 (1,8-bismaleimido-diethyleneglycol)
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 2-unit (14.7A) PEG spacer arm.
Thermo Scientific™ Sulfo-MBS (m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ Pierce™ SM(PEG)2, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)2 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ Pierce™ SM(PEG)12, No-Weigh™ Format
Thermo Scientific™ Pierce™ SM(PEG)12 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific™ Pierce™ DSG, No-Weigh™ Format
Thermo Scientific™ Pierce™ DSG is water-insoluble, homo-bifunctional N-hydroxysuccinimide ester (NHS-ester) crosslinker often used for conjugating radiolabeled ligands to cell-surface receptors.
Thermo Scientific™ MBS (m-maleimidobenzoyl-N-hydroxysuccinimide ester)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ SM(PEG)6 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.
Thermo Scientific™ SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ SMPB (succinimidyl 4-(p-maleimidophenyl)butyrate)
Crosslink amines to sulfhydryls with this mid-length crosslinker composed of NHS-ester and maleimide groups at ends of an aromatic, 10-atom (11.6A) spacer arm.
Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)
Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.
Thermo Scientific™ Pierce™ SMCC
No-Weigh™ Format
Thermo Scientific™ PMPI (p-maleimidophenyl isocyanate)
Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.