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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Invitrogen™ MultiShot™ StripWell Stbl3™ Competent Cells  DFS Item

MultiShot StripWell Stbl3 Chemically Competent E. coli cells are cloning competent cells designed for cloning direct repeats found in lentiviral expression vectors.

2

Invitrogen™ MultiShot™ FlexPlate DH5α T1R Competent Cells  DFS Item

MultiShot FlexPlate DH5α T1R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications.

3

Gibco™ FreeStyle™ CHO Expression Medium  DFS Item
GREENER_CHOICE

Animal origin-free, chemically defined, protein-free medium

Cell Line FreeStyle™ CHO-S Cells, CHO
Content And Storage Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Form Liquid
Product Type Expression Medium
Endotoxin Level ≤0.25 EU/mL, ≤ 0.25 EU/mL
Classification Chemically Defined, Protein-Free, Serum-Free
Culture Type Suspension Cell Culture
Phenol Red Indicator No Phenol Red
Product Line Freestyle, Gibco
Serum Level Serum-Free
Glutamine No Glutamine
4

Invitrogen™ Tali™ Calibration Beads  DFS Item

Align and calibrate Tali™ Image-Based Cytometer every three months

5

MilliporeSigma™ Novagen™ Rosetta™ (DE3) Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Content And Storage ≤-70°C
6

Invitrogen™ MultiShot™ Mach1™ T1R Chemically Competent E. coli Cells, 96-well plate

MultiShot Mach1™ T1R Chemically Competent E. coli Cells enable rapid, high-efficiency cloning in an automation-ready 96-well format. Fast-growing and phage-resistant, these cells support same-day transformation and streamlined high-throughput workflows.

7

Gibco™ Expi293™ Met (–) Expression Medium  DFS Item

Expi293 Met (–) Expression Medium is a cGMP-manufactured, chemically defined, serum-free, protein-free, animal origin-free, methionine-free medium for growth and transfection of Expi293F cells.

8

Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells  DFS Item

MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

9

Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli  DFS Item

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

10

MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >5 x 106 cfu/μg
11

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLysS Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

12

Invitrogen™ TOP10F' Electrocomp™ Kit  DFS Item

TOP10F' Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F' episome.

13

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Promoter Trc, Tac, Lac
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome Yes
14

Invitrogen™ MultiShot™ FlexPlate Mach1™ T1R Competent Cells  DFS Item

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

15

MilliporeSigma™ Novagen™ NovaBlue GigaSingles™ Competent Cells

NovaBlue GigaSingles™ Competent Cells produce greater than 1 x 10⁹ colonies/μg plasmid DNA for cloning applications requiring high-efficiency transformations.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >1 x 109 cfu/μg