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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1 – 15 of 93 results
1

Thermo Scientific™ DH5α Competent Cells

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

2

Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

3

Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.

4

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome No
5

MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
6

MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLacI Competent Cells

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: 2 x 106 cfu/μg
7

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Singles Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
8

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
9
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Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.

10

Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

11

Invitrogen™ One Shot™ INVαF' Chemically Competent E. coli

One Shot INVαF' Chemically Competent E. coli are designed to stably replicate high-copy number plasmids.

12

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Promoter Trc, Tac, Lac
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome Yes
13

Invitrogen™ MultiShot™ TOP10 Chemically Competent E. coli

MultiShot TOP10 Chemically Competent E. coli cells are derived from DH10B and are one of the most popular laboratory strains.

14

Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA No
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 1010 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid May be used for plasmids >20 kb
Reduces Recombination Yes (recA13)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Electrocompetent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome No
15

Invitrogen™ MultiShot™ StripWell TOP10 Chemically Competent E. coli

MultiShot StripWell TOP10 Chemically Competent E. coli cells are ideal for high-efficiency cloning and plasmid propagation, achieving a transformation efficiency of >1 x 108 cfu/μg with control plasmid DNA in StripWell format.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level Medium Efficiency (1 x 108 to 1 x 109 cfu/μg)
High-throughput Compatibility High
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Product Type Chemically Competent Cells
Product Line MultiShot
Contains F' Episome No