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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1 – 15 of 93 results
1

Thermo Scientific™ DH5α Competent Cells

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

2

Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

3

Invitrogen™ Subcloning Efficiency™ DH5α Competent Cells

Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.

4

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot™
Contains F' Episome No
5

MilliporeSigma™ Novagen™ Origami™ 2 (DE3) Competent Cells

Origami 2 strains have mutations in glutathione reducatase (gor) and thioredoxin reductase (trxB), facilitating proper disulfide bond formation.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
6

MilliporeSigma™ Novagen™ Rosetta™ 2(DE3)pLacI Competent Cells

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: 2 x 106 cfu/μg
7

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Singles Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
8

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
9

Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells

Capable of supporting site-specific recombination between pFastBac™ and bMON14272

10
Promotions Available

Invitrogen™ MegaX DH10B T1R Electrocomp™ Cells

MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.

11

Thermo Scientific™ BL21(DE3) Competent Cells

Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.

12

Thermo Scientific™ DH10B Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.

13

Invitrogen™ MAX Efficiency™ Stbl2™ Competent Cells

MAX Efficiency Stbl2 Competent Cells are high-efficiency chemically competent cells specifically designed for cloning unstable inserts.

14

Invitrogen™ MAX Efficiency™ DH5α Competent Cells

MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.

15

Invitrogen™ Library Efficiency™ DH5α Competent Cells

Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications.