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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

MilliporeSigma™ Novagen™ Rosetta™ (DE3) Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli.

Content And Storage ≤-70°C
2

Invitrogen™ One Shot™ TOP10F' Chemically Competent E. coli  DFS Item

One Shot TOP10F' Chemically Competent E. coli are identical to TOP10 cells, with the addition of an F' episome.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA Not suitable for cloning unstable DNA
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Promoter Trc, Tac, Lac
Preparing Unmethylated DNA No
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptamycin, Tetracycline)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome Yes
3

Invitrogen™ MultiShot™ StripWell DH5α T1R Competent Cells  DFS Item

MultiShot StripWell DH5α T1R chemically competent E. coli cells are cloning competent cells packaged in a rack containing 12 strips of 8 tubes to increase productivity for medium-throughput bacterial transformations.

4

MilliporeSigma™ Novagen™ Rosetta™ (DE3) pLysS Competent Cells

Rosetta host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. T7 lysozyme expression suppresses basal T7 expression.

Content And Storage ≤-70°C
5

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 Competent Cell Set

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

6

Invitrogen™ MultiShot™ FlexPlate DH10B T1R Competent Cells  DFS Item

MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

7

Invitrogen™ One Shot™ BL21-AI™ Chemically Competent E. coli  DFS Item

One Shot BL21-AI Chemically Competent E. coli cells are designed for applications that require tight regulation and strong expression of toxic proteins from any T7-promoter-based expression systems.

8

Invitrogen™ MultiShot™ FlexPlate Mach1™ T1R Competent Cells  DFS Item

MultiShot FlexPlate Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

9

MilliporeSigma™ Novagen™ RosettaBlue™ (DE3)pLysS Competent Cells

RosettaBlue host strains combine high transformation efficiency with enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >1 x 106 cfu/μg
10

MilliporeSigma™ Novagen™ Rosetta-gami™ 2 (DE3) pLysS Competent Cells

Rosetta-Gami 2 host strains allows for enhanced disulfide bond formation and enhanced expression of eukaryotic proteins. T7 lysozyme expression suppresses basal T7 expression.

11

MilliporeSigma™ Novagen™ Rosetta™ 2 (DE3) Competent Cells

Novagen's Rosetta™ 2 host strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. Coli.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 106 cfu/μg
12

Invitrogen™ One Shot™ PIR1 Chemically Competent E. coli  DFS Item

One Shot PIR1 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).

13

Invitrogen™ MultiShot™ FlexPlate TOP10 Competent Cells  DFS Item

MultiShot FlexPlate TOP10 chemically competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

14

Invitrogen™ MultiShot™ FlexPlate DH5α T1R Competent Cells  DFS Item

MultiShot FlexPlate DH5α T1R Chemically Competent E. coli cells are cloning competent cells derived from a well-known, versatile DH5α strain that can be used in many everyday cloning applications.

15

Invitrogen™ TOP10F' Electrocomp™ Kit  DFS Item

TOP10F' Electrocomp Kit E. coli cells are identical to TOP10 cells, with the addition of an F' episome.