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Suitable for rapid amplification of cDNA ends (RACE) between defined point in mRNA and the 5' end
Offers efficient and convenient method for size-fractioning double-stranded cDNA (1)
Converts up to 1μg of total RNA preparation or 100ng of control RNA into cDNA in one reaction
Illumina-compatible next-gen sequencing (NGS) libraries from as little as 50ng of starting DNA. DNA fragmentation and transposon-tagging are accomplished in one step.
Designed to amplify cDNA only from full-length, capped mRNA, usually producing a single band after PCR
Content And Storage | •FastAP™ Thermosensitive Alkaline Phosphatase |
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Format | Kit |
Reverse Transcriptase | M-MLV |
Final Product Type | cDNA |
Product Type | RLM-RACE Kit |
Quantity | 1 Kit |
Optimal Reaction Temperature | 42°C |
For Use With (Application) | cDNA Libraries & Library Construction |
Product Line | Ambionâ„¢, FirstChoiceâ„¢ |
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.
Vectors designed for expression of C-terminal-tagged HaloTagâ„¢ fusion proteins in mammalian cells. Once expressed, use the HaloTagâ„¢ fusion protein for cell imaging of protein localization or trafficking in conjunction with the fluorescent HaloTagâ„¢ Ligands.
Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker
HaloTagâ„¢ Cloning Starter Kit: designed to use Flexi Cloning System to clone protein coding sequences into mammalian HaloTagâ„¢ expression vectors; clone and transfer gene sequences into C- or N-terminus of HaloTagâ„¢ Flexi CMV Deletion Vectors.
Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream of the protein coding region of interest.
These vectors are used for inducible expression of N-terminal GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter.
Designed for high-level expression of proteins in mammalian cells from the CMV promoter without a selectable marker
The Flexi Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences.
These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied as vectors used with the Flexi Cloning System.