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Thermo Scientific™ MuSeek Library Preparation Kit, Illumina compatible
Description
Thermo Scientific MuSeek Library Preparation Kit, Illumina compatible is designed for generation of high-quality genomic DNA libraries for sequencing with the Illumina™ MiSeq™ and HiSeq™ systems. The fast protocol utilizes the MuA transposase enzyme, which catalyzes simultaneous fragmentation of double-stranded target DNA and tagging the fragment ends with transposon DNA. In a subsequent PCR step the platform-specific adapters are added using a robust and accurate Thermo Scientific™ Phusion™ High-Fidelity DNA polymerase. Starting with only 50ng or 100ng of sample the protocol can be used to generate 100 bp to 1,000 bp insert libraries. The kit contains components sufficient for 12 fragmentation reactions and the subsequent adapter-addition polymerase chain reactions. Sequencing primers required for sequencing libraries prepared by this method are provided as well.
Highlights
- Convenient – no physical shearing, end-repair and ligation steps
- Fast – generates NGS fragment libraries in less than 90 minutes
- Low sample input – DNA fragment libraries from 50ng DNA input
- Indexing compatible – MuSeek Index Set 1 enables preparation of up to 24 dual-indexed DNA libraries for sequencing in one run
- DNA fragment library preparation for downstream use in next generation sequencing workflow
- Library preparation from gDNA, ds DNA, bacterial DNA, DNA amplicons
- MuSeek Enzyme Mix, IL
- MuSeek Fragmentation Reaction Buffer
- MuSeek Stop Solution
- Water, nuclease-free
- Control DNA
- MuSeek Adapter Addition Primer Mix, IL
- MuSeek Adapter Addition Reaction Buffer, IL
- Phusion™ Hot Start II High-Fidelity DNA Polymerase, IL
- MuSeek Sequencing Primer, Read 1
- MuSeek Sequencing Primer, Read 2
- MuSeek Sequencing Primer, Index Read
The kit is shipped on dry ice. Upon arrival store at -20°C, avoid repeated freeze-thaw cycles. The Stop Solution can be stored at room temperature.
Specifications
Specifications
| Product Type | Library Preparation Kit |
| Libraries | Fragment Library |
| For Use With (Equipment) | Illumina™ HiSeq 2500, Illumina ™ MiSeq |
| Sample Type | DNA |
| Sequencing Type | Genome & DNA Sequencing |
| Workflow Step | Library Generation |
| Product Line | MuSeek |
| For Use With (Application) | NGS library preparation |
| Quantity | 12 Reactions |
Frequently Asked Questions (FAQs)
MuSeek is not suitable for ssDNA, as MuA transposase is not able to fragment ssDNA. To use MuSeek, ssDNA should first be converted into dsDNA.
Yes. However for construction of NGS library from PCR amplicons in fragmentation reaction, do not use PCR products shorter than 300 bp. Due to intrinsic features of the transposon technology, a approximately 50 bp drop off is expected in sequencing coverage from each distal end of the amplicon sequence. This can be averted by designing your amplicons to be approximately 100 bases larger than the desired sequencing insert.
No, the recommended sample DNA input should be followed strictly. The MuSeek fragmentation reaction is optimized to yield optimal fragment library lengths at recommended input.
We do not supply specific MuSeek kits for NGS library preparation from RNA samples. However, MuSeek is compatible with double-stranded cDNA substrate.
The ClaSeek technology uses a classical NGS library preparation approach where physically fragmented DNA is end-repaired and platform-specific adapters are added through ligation reaction. MuSeek transposase-based technology simultaneously fragments intact DNA and adds MuA transposon derived DNA sequence which is further used for PCR-based adapter addition step.
For Research Use Only. Not for use in diagnostic procedures.