Cell Based Assays
Invitrogen™ XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)
Redox-based viability probe
Invitrogen™ PrestoBlue™ and PrestoBlue™ HS Cell Viability Reagents
Monitor cell proliferation and viability with the resazurin-based PrestoBlue and PrestoBlue HS cell viability reagents. These nontoxic and cell permeable reagents quantitatively measure cell viability in as little as 10 minutes using absorbance- or fluorescence-based microplate readers.
Molecular Probes™ MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
Invitrogen™ LIVE/DEAD™ BacLight™ Bacterial Viability Kits
LIVE/DEAD™ BacLight™ Bacterial Viability Kits are easy-to-use fluorescent staining kits commonly used for assessing the viability of bacterial cells. These kits provide a two-color fluorescence assay of bacterial viability using green and red fluorescent dyes for use with imaging, flow, and assays.
Invitrogen™ Calcein AM, Cell-permeant Green and Blue Dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Invitrogen™ DAPI and Hoechst Nucleic Acid Stains
DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.
Molecular Probes™ Annexin V Conjugates for Apoptosis Detection
Detect early stages of apoptosis with Annexin V stand-alone Alexa Fluor, APC, Pacific Blue, PE, FITC, and biotin conjugates using flow cytometry.
Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Useful with a variety of fluorescence-detection methodologies
Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
MilliporeSigma™ Acetyl-Histone H4 Immunoprecipitation (ChIP) Assay Kit
Used to immunoprecipitate transcriptionally active chromatin from mammalian cells using anti-Acetyl-Histone H4, ChIP grade rabbit antiserum
MilliporeSigma™ Anti-Cdc42 Immunoblotting Kit
Detects Cdc42 when performing western blots
MilliporeSigma™ Upstate™ Histone Deacetylase Assay Kit (HDAC)
For enzyme assay
MilliporeSigma™ Rac1 Activation Assay Kit
Used to precipitate Rac1-GTP from cell lysates & detection by a Rac1 specific monoclonal antibody
MilliporeSigma™ Chromatin Immunoprecipitation (ChIP) Assay Kit
Used to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types
MilliporeSigma™ EZ-Magna ChIP™ A - Chromatin Immunoprecipitation Kit
Single day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads