Cell Based Assays
Invitrogen™ Total Reactive Oxygen Species (ROS) Assay Kit 520 nm
Contains the necessary regent and buffer for identifying ROS in cells by flow cytometry in the FITC channel. Reactive oxygen species are chemically reactive oxygen-containing molecules that are generated as a natural byproduct of the oxygen metabolism.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 455UV
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ BrdU Staining Buffer Set for Flow Cytometry
Contains the buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set, in combination with BrdU and DNase I steps, allows for detection of BrdU pulse-labeled cells, thereby enabling the examination of proliferating cells.
Invitrogen™ CellVue™ Maroon Cell Labeling Kit
Far-red fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ NIR815 Cell Labeling Kit
Near-infrared fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ Burgundy Cell Labeling Kit
Far-red/near-infrared fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ Plum Cell Labeling Kit
Far-red fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling is rapid and stable and can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ Jade Cell Labeling Kit
Green fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ CellVue™ Lavender Cell Labeling Kit
Violet fluorescent reagent used to label the cell membrane in order to identify and track labeled cells. Cell labeling can be combined with fluorescently labeled antibodies and other markers of cellular function for flow cytometric analysis and fluorescent microscopy.
Invitrogen™ eBioscience™ CFSE
Widely used for cell tracking and proliferation studies. It has also been used in CTL assays and cell motility studies. CFSE readily crosses intact cell membranes.
Invitrogen™ eBioscience™ JC-1 Mitochondrial Membrane Potential Dye
Used to determine mitochondrial membrane potential in flow cytometry and fluorescent microscopy. Dye selectively enters mitochondria where it reversibly changes color as membrane potentials increase. Property due to the reversible formation of JC-1 aggregates upon membrane polarization.
Invitrogen™ eBioscience™ Calcein AM Viability Dye (UltraPure Grade)
A membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein fluorescent dye.
Invitrogen™ eBioscience™ Indo-1 AM Calcium Sensor Dye (UltraPure Grade)
Membrane-permeable dye used for determining changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.