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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

EDGE
Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
2

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

EDGE
3

Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

EDGE
Content And Storage Store frozen
Sterilization Method 0.1 μm Sterile-filtered
Form Liquid
Shelf Life 24 Months
Source Porcine pancreas
4

MilliporeSigma™ PluriSTEM™ Dispase-II Solution

a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.

5

MilliporeSigma™ Trypsin 0.25% and 1mmol EDTA

The Trypsin-EDTA, In Hank's Balanced Salt Solution, 0.25% Trypsin and 1mM EDTA, without Ca 2+ and Mg 2+ is available in a 100mL format.

8

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

9

MilliporeSigma™ Enzyme Free Cell Dissociation Solution Hank's Based (1x)

Hank's based formulation of chelating agents and agents to stabilize their activity on cells

Content And Storage 4°C
Formulation Chelating and stabilizing agents
10

MilliporeSigma™ Xeno-Free 0.05% Trypsin/0.02% EDTA Kit

Useful for routine detachment of cells from standard tissue plasticware and adhesion coated plasticware under animal-free conditions..

11

MilliporeSigma™ Calbiochem™ Trypsin Porcine MS Grade

A chemically modified trypsin that is resistant to autolysis and degradation

12

Gibco™ TrypLE™ Select Enzyme (10X), no phenol red
GREENER_CHOICE

TrypLE™ Select Enzyme (10X), no phenol red

Chelators EDTA
Purity or Quality Grade Cell Culture Grade
Inorganic Salts No Calcium, No Magnesium, No Sodium Phosphate
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 10 X
Osmolality 270 - 320 mOsm/kg
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
pH 6.9 to 7.4
Shelf Life 24 Months
Product Line Gibco
13

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red  DFS Item
GREENER_CHOICE

Unlike porcine trypsin, free of animal-derived components

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Without Additives No Phenol Red
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
14

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

15

Cytiva HyrTrp Cell Dissociation Reagent

Recombinant and animal-derived component-free alternative to traditional porcine trypsin

Content And Storage -20 °C
For Use With Attachment-dependent cell types MRC-5, Vero, CHO K-1 cell lines grown with or without serum
Product Type Cell Culture Dissociation Reagent
Shelf Life 12 months from manufacturing date when stored at -20°C