Standard PCR Reagents and Kits

Standard PCR Reagents and Kits

Products for use during standard PCR procedures. Includes master mixes, DNA polymerases, reverse transcription mixes, PCR beads, buffers, and kits. Ideal when specificity; precise quantification; and high fidelity, sensitivity, and yield are not requirements.
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1 – 30 of 52 results
1

Fisher BioReagents™ exACTGene™ Control Primers

Optimized for routine PCR applications

2

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Includes Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
3

Invitrogen™ Taq DNA Polymerase, native  DFS Item

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Format Stand-alone enzyme
Concentration 5 U/µL
Starting Material DNA
PCR Method PCR
Detection Method Primer-Probe
4

Invitrogen™ Taq DNA Polymerase PCR Buffer (10X)  DFS Item

A 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl]

5

Invitrogen™ PCR SuperMix  DFS Item

Ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification

6

Thermo Scientific™ ThermoPrime Taq DNA Polymerase, with 10x buffer and separate vial of 25 mM MgCl2

For PCR applications requiring increased yield and sensitivity, these PCR kits and master mixes are optimized to produce large amounts of PCR product.

7

Invitrogen™ Taq DNA Polymerase, recombinant  DFS Item

A thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer

Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Reaction Format Separate Components
Hot Start No
Overhang 3'-A
Concentration 5 U/µL
8

Invitrogen™ SuperScript™ IV RT-LAMP Master Mix  DFS Item

Invitrogen SuperScript IV RT-LAMP Master Mix is a solution designed for fast, sensitive, and simple RNA target detection in loop-mediated isothermal amplification (LAMP) reactions.

Polymerase Bst DNA Polymerase
Reverse Transcriptase SuperScript™ IV
Product Type Master Mix
Reaction Speed 5 to 15 minutes
Optimal Reaction Temperature 65°C
9

Applied Biosystems™ GeneAmp™ 10X PCR Buffer I, (2 x 75 mL)  DFS Item

Optimized for use with AmpliTaq™ DNA Polymerase for robust PCR amplification

10

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase  DFS Item

Amplifies vast range of DNA sequence contexts when used with enhanced AmpliTaq 360 Buffer and 360 GC Enhancer

Format Stand-alone enzyme
Overhang 3'-A
Concentration 5 U/µL
Starting Material DNA
11

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Polymerase AmpliTaq™ DNA Polymerase
Speed Fast, Standard
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Dye Type Passive Reference Dye: ROX (Pre-mixed)
Detection Method Primer-Probe Detection
12

Applied Biosystems™ AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer  DFS Item

Use with AmpliTaq 360 DNA polymerase to supplement reagents for experiments when needed

13

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20mL Sample prep, 50mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Polymerase AmpliTaq™ DNA Polymerase
Speed Fast, Standard
Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Dye Type Passive Reference Dye: ROX (Pre-mixed)
Detection Method Primer-Probe Detection
14

Applied Biosystems™ TaqMan™ DNA Template Reagents  DFS Item

Provide the necessary components to perform a standard-dilution series with the beta-actin gene using nucleic acid from human cells

Promotions are available
15

Applied Biosystems™ GeneAmp™ Fast PCR Master Mix (2X), with protocol  DFS Item

Sensitive, pre-mixed hot-start chemistries

Format Tube
Product Type Fast PCR Master Mix
Hot Start Built-In Hot Start
Exonuclease Activity 5' - 3'
16

Cytiva RAPD Analysis Beads

For performing DNA-profiling experiments using the randomly amplified polymorphic DNA (RAPD) technique

No. of Reactions 100
Format 0.5mL Tube
Volume 500μL
Color Colorless
pH 8.3
Quantity 1 Kit
For Use With (Application) For performing DNA profiling experiment
17

Cytiva Hot Start Master Mix

A ready-to-use 2X mastermix providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

18

Cytiva Taq DNA Polymerase (cloned)

For PCR and other procedures requiring DNA polymerase activity at elevated temperatures

Includes 100mM Tris HCl, pH 9, 15mM MgCl2 and 500mM KCl
Color Colorless
Purity Free from detectable nonspecific nuclease and Taq I restriction endonuclease activities.
pH 7.5
Storage Requirements -20°C
Concentration 5000U/mL
For Use With (Application) For PCR and other procedures requiring DNA Polymerase activity at elevated temperatures
19

Cytiva PuReTaq Ready-To-Go™ PCR Beads

Pre-mixed, pre-dispensed reaction beads optimized for performing standard PCR amplifications.  Cytiva Lifescience™ illustra™ PuReTaq Ready-To-Go™ PCR Beads are stable at ambient temperature.

Promotions are available
Includes Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25μL final volume, the concentration of each dNTP
Color White
Quantity 1 Kit
Storage Requirements Ambient temperature in pouch with desiccant
For Use With (Application) For PCR application
20

Cytiva Ready-To-Go™ RT-PCR Beads

Optimized for performing either one-step or conventional two-step reverse transcription.  Cytiva Lifescience™ Illustra™ Ready-To-Go™ RT-PCR Beads are ambient temperature stable, pre-mixed, and ready for pre-dispensed reactions.

Includes Room temperature stable beads containing Buffer, dATP, dCTP, dGTP, dTTP, approximately 2 units of Taq DNA Polymerase, M-MLV reverse Transcriptase, RNAguard (porcine) and stabilizers (including RNase/DNase free BSA), When a bead is reconstituted to 50
Quantity 1 Kit
Storage Requirements Ambient in pouch with desiccant
For Use With (Application) For performing single tube one step reverse transcription PCR
21

Cytiva Hot Start Mix RTG

Premixed, predispensed, ambient temperature-stable formulation providing robust and reliable performance for demanding PCR applications in which high specificity and high sensitivity are essential to success

Includes 1.75 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer
Format Tube
Storage Requirements Ambient temperature in pouch with desiccant
For Use With (Application) Standard PCR
22

Thermo Scientific™ DreamTaq Green DNA Polymerase (5 U/µL)

Save time in standard PCR applications with this enhanced Taq DNA polymerase, supplied with colored buffer enabling direct gel loading.

Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1 X
Reaction Format Standalone
Reaction Speed Standard
Hot Start No
Overhang 3'-A
Product Line DreamTaq™
23

Thermo Scientific™ Taq DNA Polymerase, recombinant (5 U/µL)

Optimize routine PCR with this highly thermostable DNA polymerase.

24

Thermo Scientific™ DreamTaq DNA Polymerase (5 U/µL)

Get higher sensitivity, longer PCR products and higher yields in all standard PCR applications with this enhanced Taq DNA polymerase.

25

Thermo Scientific™ DreamTaq Green PCR Master Mix (2X)

Save time in PCR applications with Master Mix and get higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase.

26

Thermo Scientific™ Taq DNA Polymerase, recombinant (1 U/µL)

Optimize routine PCR with this highly thermostable DNA polymerase.

27

Thermo Scientific™ Taq DNA Polymerase, native, without BSA (5 U/µL)

Optimize amplification of DNA samples of low purity with this highly thermostable, native DNA polymerase for routine PCR.

28

Thermo Scientific™ PCR Master Mix (2X)

Optimize routin PCR with this highly thermostable DNA polymerase.

29

Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer I  DFS Item

Ultrapurified AmpliTaq DNA Polymerase ensures residual bacterial DNA sequences are substantially reduced

30

Applied Biosystems™ AmpliTaq™ DNA Polymerase, LD (Low DNA) with Buffer II  DFS Item

Ultrapurified AmpliTaq DNA Polymerase ensures residual bacterial DNA sequences are substantially reduced