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  7. Thermo Scientific™ PCR Master Mix (2X)

Thermo Scientific™ PCR Master Mix (2X)

Catalog No. FERK0172
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No. of Reactions:
1000 Reactions
200 Reactions
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FERK0172 1000 Reactions
FERK0171 200 Reactions
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Catalog No. FERK0172 Supplier Thermo Scientific™ Supplier No. K0172
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Description

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Convenient, ready-to-use mix
  • Thermostable—half life is more than 40 min. at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Note

The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

Specifications

Concentration 2X
Content And Storage • 2X PCR Master Mix, 20 x 1.25 mL
• Nuclease-free water, 20 x 1.25 mL

Store at -20°C.
GC-Rich PCR Performance Low
Polymerase Taq DNA Polymerase
Reaction Speed Standard
Product Type PCR Master Mix
Quantity 1,000 reactions
Shipping Condition Dry Ice
For Use With (Application) Standard PCR
Fidelity (vs. Taq) 1X
Hot Start No
No. of Reactions 1000 Reactions
Overhang 3'-A
Reaction Format SuperMix or Master Mix
Starting Material DNA
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Frequently Asked Questions (FAQs)

Can I do PCR directly from bacterial colonies with Thermo Scientific Taq Master Mixes?

Dream Taq PCR Master Mix and Taq PCR Master Mix can be used for direct PCR from bacterial colonies. Simply scrape a tiny amount of a single colony and transfer to a PCR-tube, prefilled with the master mix. Always use at least 3 min at 95 degrees C for initial denaturation.

For Research Use Only. Not for use in diagnostic procedures.