Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay
The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.
Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer
The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kit, 100 reactions
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
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Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kit, with PrepSeq™ sample preparation, 100 Reactions
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Applied Biosystems™ TaqMan™ Vaginal Microbiota Amplification Control
The TaqMan™ Vaginal Microbiota Amplification Control is designed to evaluate the amplification efficacy of the TaqMan Vaginal Microbiota assays. This plasmid serves as a positive control for the TaqMan Vaginal Microbiota Assay collection.
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kit, 100 reactions
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.
Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits
The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kit with PrepSEQ™ Residual DNA Sample Preparation Kit, 100 reactions
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.
Applied Biosystems™ resDNASEQ™ Quantitative E1A DNA Fragment Length Kits
The resDNASEQ Quantitative E1A DNA Fragment Length Kit is a quantitative PCR (qPCR)-based system that measures and quantitates the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines.
Applied Biosystems™ Path-ID™ Multiplex One-Step Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Applied Biosystems™ TaqMan™ SARS-CoV-2, Flu A/B, RSV RNA Control
The TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control contains targets specific to the SARS-CoV-2, influenza A/B, and RSV genomic regions targeted by the TaqMan SARS-CoV-2, FluA/B RSV RT-PCR Assay Kit.
Applied Biosystems™ ViralSEQ™ dPCR Lentivirus Proviral Titer Kit
The Applied Biosystems ViralSEQ dPCR Lentivirus Proviral DNA Titer Kit provides a wide dynamic range and high sensitivity for accurate detection of integrated provirus copies.