Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ PureQuant CD3+ T Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Applied Biosystems™ QuantStudio™ 3D Digital PCR Master Mix
Optimized for use with isolated DNA or cDNA, TaqMan™ assays, and the QuantStudio™ 3D Digital PCR Chip
Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control
The Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays included in the TaqCheck™ SARS-CoV-2 Fast PCR Assay.
Applied Biosystems™ Precision Plate Holder for Plates, for 7500 Fast System
Precision plate holder for 7500 fast system
Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.
Applied Biosystems™ resDNASEQ™ dPCR E1A DNA Fragment Length Kit
The resDNASEQ dPCR E1A DNA Fragment Length Kit is an absolute quantitation PCR-based system that measures the fragment lengths of residual host-cell DNA by targeting the E1A gene in HEK 293 cell lines, which are predominantly used in the development of gene therapies, cell-based vaccines, and similar biotherapeutics.
Applied Biosystems™ TaqMan™ Arbovirus Triplex Control Kit (ZIKV/DENV/CHIKV)
The Applied Biosystems™ TaqMan Zika Virus Triplex Control Kit (ZIKV/DENV/CHIKV) includes a single foil pouch that contains human DNA at 1X and pooled complete genome RNA template of multiple viruses.
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Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Applied Biosystems™ resDNASEQ™ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kits
The resDNASEQ Quantitative Plasmid DNA - Kanamycin Resistance Gene Kit is a quantitative PCR (qPCR)-based system for the detection of residual kanamycin-resistant plasmids.
Applied Biosystems™ resDNASEQ™ Quantitative E. coli DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.