Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ VetMAX™ Xeno™ Internal Positive Control - VIC™ Assay
The VetMAX™ Xeno™ Internal Positive Control (IPC) - VIC™ Assay is a primer-probe mix that detects the Xeno internal positive control. The resultant Xeno data is used to determine the validity of diagnostic test results.
Applied Biosystems™ QuantStudio™ Absolute Q™ Isolation Buffer
The QuantStudio Absolute Q Isolation Buffer is intended to supplement the materials supplied in the QuantStudio Absolute Q MAP16 Plate Kit.
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.
Invitrogen™ TaqMan™ Cells-to-CT™ HepatoExpress™ Kit
The TaqMan Cells-to-CT HepatoExpress Kit is a complete cell lysate system designed for gene expression analysis directly from hepatocytes without the need of RNA purification.
Applied Biosystems™ Megaplex™ Primer Pools, Rodent Pools Set v3.0
Megaplex™ Primer Pools, Rodent Pools Set v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools Sets.
Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits
The resDNASEQ™ Quantitative NS0 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines.
VetMAX™-Gold AIV Detection Kit
The VetMAX™-Gold AIV Detection Kit (Avian Influenza Virus RNA Test Kit) is a highly sensitive, qualitative, one-step, real-time RT-PCR assay to detect Avian Influenza Virus (AIV) RNA isolated from poultry (chicken/turkey) oropharyngeal/tracheal swab samples.
Applied Biosystems™ SteriSEQ™ Rapid Sterility Testing Kit
The SteriSEQ Rapid Sterility Testing Kit is a TaqMan-based quantitative PCR (qPCR) kit for the detection of bacteria and fungi in complex bioproduction samples.
Thermo Scientific™ SureTect™ Beverage Spoilage Multiplex PCR Assay
Rapid qPCR solutions for beverage manufacturers. Rapidly detect a broad range of beverage spoilers from one PCR reaction with this assay.
Applied Biosystems™ ViralSEQ™ Mouse Minute Virus (MMV) Detection System
The Applied Biosystems ViralSEQ Mouse Minute Virus (MMV) Detection Assay is part of an integrated DNA purification and real-time PCR-based system for rapid, sensitive detection of MMV from a wide range of sample types.
Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
Applied Biosystems™ QuantStudio™ 12K Flex OpenArray™ Vaginal Microbiota Starter Kit
The QuantStudio™ 12K Flex OpenArray™ Vaginal Microbiota Starter Kit contains two training plates and a synthetic amplification control to serve as a sample for experiments.
Applied Biosystems™ TaqMan™ Arbovirus Triplex Control Kit (ZIKV/DENV/CHIKV)
The Applied Biosystems™ TaqMan Zika Virus Triplex Control Kit (ZIKV/DENV/CHIKV) includes a single foil pouch that contains human DNA at 1X and pooled complete genome RNA template of multiple viruses.