Hot Start PCR Reagents and Kits

Hot Start PCR Reagents and Kits
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Thermo Scientific™ Phire Hot Start II DNA Polymerase
Thermo Scientific Phire Hot Start II DNA Polymerase is an enhanced PCR enzyme for routine and high throughput PCR applications. It is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | Separate Components |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mixes
Thermo Scientific DreamTaq Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs.
Shipping Condition | Dry Ice |
---|---|
Polymerase | DreamTaq DNA Polymerase |
GC-Rich PCR Performance | Low |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 2X |
For Use With (Application) | Hot-start PCR |
Thermo Scientific™ DreamTaq™ Hot Start DNA Polymerase
Thermo Scientific DreamTaq Hot Start DNA Polymerase is an enhanced hot start Taq DNA polymerase that enables higher PCR specificity, sensitivity, and yields compared to conventional hot start Taq DNA polymerases.
Polymerase | DreamTaq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Invitrogen™ Platinum™ II Green PCR Buffer (5X)
Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
Polymerase | AmpliTaq Gold DNA Polymerase |
---|---|
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 5 kb or less |
Product Type | DNA Polymerase |
Overhang | 3'-A |
Concentration | 5 U/μL |
Exonuclease Activity | 5' - 3' |
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
Polymerase | Platinum Taq DNA Polymerase |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Starting Material | DNA |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Thermo Scientific™ Phire Hot Start II PCR Master Mixes
Thermo Scientific Phire Hot Start II PCR Master Mix is convenient 2X mix designed to minimize the number of pipetting steps. The master mix contains Phire Hot Start II DNA Polymerase, nucleotides and optimized reaction buffer including MgCl2.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Phire Hot Start II DNA Polymerase |
GC-Rich PCR Performance | Medium |
Fidelity (vs. Taq) | 2X |
Reaction Format | SuperMix or Master Mix |
Product Type | Hot Start PCR Master Mix |
Reaction Speed | Fast |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
Concentration | 2X |
Starting Material | DNA |
For Use With (Application) | Cloning,Hot-start PCR |
Applied Biosystems™ Platinum™ Multiplex PCR Master Mix
The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Platinum Taq DNA Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 2.5 kb or less |
Reaction Format | SuperMix or Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Concentration | 2X |
For Use With (Application) | Hot-start PCR,Multiplex PCR |
Multiplex Capability | Up to 20-plex |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
GC-Rich PCR Performance | Low |
---|---|
Fidelity (vs. Taq) | 1X |
Product Type | DNA Polymerase With Buffer I |
Hot Start | Built-In Hot Start |
Overhang | 3'-A |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Invitrogen™ Platinum™ GenoType Tsp DNA Polymerase
Platinum GenoType Tsp DNA Polymerase is designed and qualified specifically for amplification of dinucleotide repeat markers in PCR-based genotyping applications.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | Tsp Genotyping Polymerase |
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 1X |
Size (Final Product) | 500 bp or less |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR |
Invitrogen™ AccuPrime™ Taq DNA Polymerase System
The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.
Shipping Condition | Wet or Dry Ice |
---|---|
GC-Rich PCR Performance | Low |
Fidelity (vs. Taq) | 2X |
Overhang | 3'-A |
Concentration | 10X |
For Use With (Application) | Hot-start PCR |
PCR Method | 1-step RT-qPCR,Multiplex qPCR |
Detection Method | Primer-probe |
Polymerase | AccuPrime Taq DNA Polymerase |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase System |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase
Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase with a 10X Green PCR Buffer.
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
Shipping Condition | Wet or Dry Ice |
---|---|
Polymerase | AccuPrime GC-Rich DNA Polymerase |
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2X |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Overhang | Blunt |
For Use With (Application) | Hot-start PCR |
Applied Biosystems™ AmpliTaq Gold™ 360 Master Mix
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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AmpliTaq Gold 360 PCR Master Mix contains everything required for successful PCR amplification in one convenient package–all components are premixed and premeasured.
Shipping Condition | Room Temp or Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 2X |
For Use With (Application) | Cloning,Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | SuperMix or Master Mix |
Product Type | PCR Master Mix |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |
Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More

Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.
Shipping Condition | Room Temp or Wet Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 1X |
Overhang | 3'-A |
Green Features | Sustainable packaging |
Concentration | 5 U/μL |
For Use With (Application) | Hot-start PCR |
Exonuclease Activity | 5' - 3' |
Polymerase | AmpliTaq Gold 360 DNA Polymerase |
Format | Tube |
Size (Final Product) | 5 kb or less |
Reaction Format | Separate Components |
Product Type | DNA Polymerase |
Reaction Speed | Standard |
Hot Start | Built-In Hot Start |
Starting Material | DNA |