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Invitrogen™ Platinum™ Taq Green Hot Start DNA Polymerase
Description
Invitrogen Platinum Taq Green Hot Start DNA Polymerase provides Platinum Taq DNA Polymerase and a 10X Green PCR Buffer. Platinum Taq DNA Polymerase is a convenient and reliable hot-start thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase. The hot start property of the enzyme is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step, thus preventing the extension of nonspecifically annealed primers and improving product yield.
Platinum Taq DNA Polymerase is a recombinant Taq DNA polymerase complexed with proprietary Platinum antibodies. The antibodies dissociate during the initial PCR denaturation step and the DNA polymerase regains its full activity. Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a non-template-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends and has a 5' to 3' exonuclease activity.
Platinum Taq Green Hot Start DNA Polymerase is provided with Green PCR Buffer that contains a density reagent and two tracking dyes and allows for direct gel loading of PCR products. The optional KB Extender used with Platinum Taq DNA Polymerase enables more versatility in PCR assays with long or GC-rich amplicons. Use Platinum Taq Green Hot Start DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR.
Features
- High specificity and increased yields with antibody-mediated hot-start PCR
- Convenient room-temperature reaction setup
- Direct gel loading with green PCR buffer
- Versatile formulation for broad range of amplicons
Notes
- The next-generation enzyme Platinum II Taq Hot-start DNA Polymerase is recommended for superior performance.
- Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
Specifications
Specifications
| Content And Storage | • Platinum Taq DNA Polymerase, 120 μL • 10X Green PCR Buffer (without magnesium), 3 x 1.25 mL • 50 mM MgCl2, 1.25 mL • KB Extender, 1.25 mL Store at -20°C in a non-frost-free freezer. |
| GC-Rich PCR Performance | Low |
| Polymerase | Platinum Taq DNA Polymerase |
| Reaction Speed | Standard |
| Quantity | 600 reactions |
| Shipping Condition | Dry Ice |
| Fidelity (vs. Taq) | 1X |
| Hot Start | Built-In Hot Start |
| No. of Reactions | 600 Reactions |
| Overhang | 3'-A |
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Frequently Asked Questions (FAQs)
We have the following recommendations:
- Increase the extension time from 1 min/kb to 1.5 min/kb.
- Vary total PCR cycles from 20-40; 35 cycles is typical.
- Try denaturing at 95 degrees C for 45 seconds.
- Increase the amount of template for targets >5 kb.
- Use 2.5 U of Platinum Taq for each 50 µL reaction.
- GC-rich or problematic targets work better with MgSO4 instead of MgCl2.
- For GC-rich templates, test higher annealing temperatures starting with the temperature that is equal to your primer Tm and in increments of 2 degrees C for up to six different temperatures.
- Vary the KB extender solution from 1.5 µL to 4.5 µL per 50 µL reaction.
The KB Extender enhances the amplification of GC-rich and problematic sequences, and the extension of genomic DNA targets of >5 kb. The KB Extender lowers DNA melting temperature (Tm), and the co-solvent and amplification buffer offer higher primer specificity, broader Mg concentration and annealing temperature optima, as well as improved Taq thermostabilization. Please note that primer sets that generate specific products using standard PCR buffer may not benefit from using KB Extender. Excessive use of the KB Extender may reduce yield, particularly for non-GC rich amplicons. We generally recommend varying the KB Extender solution from 1.5-4.5 µL per 50 µL reaction.
Yes the green dye is compatible with downstream applications such as fluorescent automatic DNA sequencing, ligation, and restriction digestion.
Yes, we offer several Platinum Taq DNA polymerases and their master mixes, supplemented with tracking dyes for direct loading of PCR products on gels:
- Platinum II Hot-Start Green PCR Master Mix (2X)
- Platinum SuperFi Green DNA Polymerase and Platinum SuperFi Green PCR Master Mix
- Platinum Taq Green Hot-Start DNA Polymerase and Platinum Green Hot-Start PCR Master Mix (2X)
- DreamTaq Green Hot-Start DNA Polymerase and DreamTaq Hot-Start Green PCR Master Mix
- Phusion Green Hot-Start II High-Fidelity DNA Polymerase and Phusion Green Hot-Start II High-Fidelity PCR Master Mix.
For Research Use Only. Not for use in diagnostic procedures.