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Invitrogen™ Platinum™ II Green PCR Buffer (5X)

Catalog No. 14966123
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14966123 5 mL
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Catalog No. 14966123 Supplier Invitrogen™ Supplier No. 14966123
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Description

Includes

4 x 1.25 ml 5X Platinum II Green PCR buffer

Invitrogen 5X Platinum II Green PCR Buffer for fast and easy PCR

Invitrogen Platinum II Green PCR Buffer is an innovative buffer optimized for use with Platinum II Taq Hot-Start DNA Polymerase. Due to its unique composition, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair.

Platinum II Green PCR Buffer provides 1.5 mM MgCl2 in the final reaction and is supplemented with a density reagent and two tracking dyes for direct loading of PCR products on gels.

Specifications

Concentration 5X
Content And Storage 5X Platinum II Green PCR buffer, 4 x 1.25 mL

Store at -20°C in a non-frost-free freezer.
Format Tube
Polymerase Platinum II Taq Hot-Start DNA Polymerase
Reaction Speed Fast
Product Type PCR Buffer
Quantity 5 mL
Volume 4 x 1.5 mL

Frequently Asked Questions (FAQs)

Do the dyes in Platinum II Green PCR Buffer interfere with fragment separation of the PCR product on E-Gel agarose gels?

No. The tracing dyes (a blue and a yellow dye) in Platinum II Green PCR Buffer do not interfere with fragment separation on E-Gel agarose gels.
Do the dyes in Platinum II Green PCR Buffer interfere with PCR performance of Platinum II Taq Hot-Start DNA Polymerase?

No. The tracing dyes (a blue and a yellow dye) in Platinum II Green PCR Buffer do not interfere with PCR performance and do not change any enzyme features.

For Research Use Only.