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Applied Biosystems™ Platinum™ Multiplex PCR Master Mix

Catalog No. 4464270
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Description

Includes

Platinum Multiplex PCR Master Mix, 2X; GC Enhancer

The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.

Platinum Multiplex PCR Master Mix is a high-specificity, high-throughput solution designed specifically for endpoint multiplex PCR and supports easy multiplexing with minimal optimization.

The performance of the Platinum Multiplex PCR Master Mix over a wide range of amplicon sizes permits the amplification of templates from 50 bp to 2.5 kb, greatly enhancing workflow flexibility. Coupled with its 20-plex capability and absence of primer dimers, it not only provides a high-throughput solution but also boasts high specificity through fewer non-specific primer binding events, resulting in less reaction and primer waste.

Features

  • Amplifies up to 20 amplicons in a single reaction
  • Amplifies products from 50 bp to 2.5 kb
  • Performs multiplex PCR reactions with minimal optimization

Order Info

Shipping Condition: Dry Ice

Specifications

Concentration 2X
Content And Storage • Platinum Multiplex PCR Master Mix, 2X (5 x 10 mL)
• GC Enhancer (1 x 10 mL)

Store at –15°C to –25°C prior to opening.
Master mix may be stored at –15°C to –25°C or at 4°C (for up to 30 days). The GC Enhancer must be kept at –15°C to –25°C.
GC-Rich PCR Performance Low
Polymerase Platinum Taq DNA Polymerase
Reaction Speed Standard
Multiplex Capability Up to 20-plex
Quantity 2000 Reactions
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR, Multiplex PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 2000 Reactions
Reaction Format SuperMix or Master Mix
Size (Final Product) 2.5 kb or less
Volume 10 mL
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Frequently Asked Questions (FAQs)

Does the Platinum Multiplex PCR master mix leave A overhangs?

Yes, the Platinum Multiplex PCR master mix contains Platinum Taq DNA Polymerase that leaves A overhangs on PCR products.

What is the difference between Platinum technology and AccuPrime technology?

With Platinum technology, anti-DNA polymerase antibodies bind to the enzyme until the denaturing step at 94 degrees C, when the antibodies degrade. The polymerase is now active and primer extension can occur. AccuPrime Taq combines Platinum Taq (Taq + Platinum antibodies) with proprietary thermostable AccuPrime accessory proteins. The 10X reaction buffer contains the accessory proteins which enhance specific primer-template hybridization during each cycle of PCR.

Can I use Platinum Multiplex PCR Master Mix for amplifying uracil-containing templates?

Yes, Platinum Multiplex PCR Master Mix contains Platinum Taq Polymerase that can incorporate dUTP and amplify uracil-containing templates.

For Research Use Only. Not for use in diagnostic procedures.