missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. PCR Equipment and Supplies
  4. PCR Supplies
  5. Standard PCR Products
  6. Hot Start PCR Reagents and Kits
  7. Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2

Numéro de catalogue. N8080259
Encompass
Change view
Click to view available options
Quantity:
1,000 units
25 x 1,000 units
250 Units
3,000 units
5,000 units
5 product options available for selection
Product selection table with 5 available options. Use arrow keys to navigate and Enter or Space to select.
Numéro de catalogue. Quantity
N8080259 25 x 1,000 units
N8080241 250 Units
N8080247 1,000 units
N8080245 3,000 units
N8080249 5,000 units
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 5 options available.
5 options
Numéro de catalogue. N8080259 Fournisseur Applied Biosystems™ Code fournisseur N8080259

Pour de laide SVP utiliser la function de clavardage; nous envoyer un couriel à help@thermofisher.com ou appelez le service à la clientèle au 1-800-234-7437.

Description

AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.

AmpliTaq Gold DNA Polymerase with Buffer II and MgCl2 is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation. The modified enzyme is provided in an inactive state and upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

AmpliTaq Gold DNA Polymerase can be activated partially or completely in a pre-PCR heat step or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases as reactants are not wasted in the formation of unintended products.

Features

  • Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
  • Time-released thermal activation improves sensitivity in low copy number amplifications
  • Successful multiplex PCR saves time and reagents
  • Includes GeneAmp 10X PCR Buffer II with MgCl2
  • Hot-start technology reduces risk of biological contamination

Notes

  • For better performance AmpliTaq Gold 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation.
  • When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates.

Order Info

Shipping condition: Dry Ice

Spécifications

Concentration 5 U/μL
Content And Storage • AmpliTaq Gold DNA Polymerase (5 U/μL), 25 x 200 μL
• 10X PCR Buffer II, 100 x 1.5 mL
• 25 mM MgCl2, 100 x 1.5 mL

Store at -15°C to -30°C.
Format Tube
GC-Rich PCR Performance Low
Polymerase AmpliTaq Gold DNA Polymerase
Reaction Speed Standard
Exonuclease Activity 5' - 3'
Product Type DNA Polymerase
Quantity 25 x 1,000 units
Shipping Condition Dry Ice
For Use With (Application) Hot-start PCR
Fidelity (vs. Taq) 1X
Hot Start Built-In Hot Start
No. of Reactions 20,000 Reactions
Overhang 3'-A
Reaction Format Separate Components
Size (Final Product) 5 kb or less
Starting Material DNA
Afficher plus de résultats Afficher moins de résultats

For Research Use Only. Not for use in diagnostic procedures.