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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Invitrogen™ Cells-to-CT™ Stop Solution  DFS Item
GREENER_CHOICE

A component of the Cells-to-CT™ Kits.

2

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.

3

Invitrogen™ Cells-to-cDNA™ II Kit  DFS Item

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Shipping Condition Dry Ice
Content And Storage 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at
-20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase M-MLV
Size (Final Product) 7 kb or less
Final Product Type cDNA
Sample Type Mammalian Cells
Purification Time 2 hr.
Optimal Reaction Temperature 42°C
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Product Line Ambion, Cells-to-cDNA
4

Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
PCR Method 2-step RT-qPCR
Detection Method Primer-probe
Polymerase DNA Polymerase
Format Tube
Product Type Gene Expression Cells-to-C Kit
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Cell
Reaction Speed Standard
Product Line Ambion, Cells-to-CT, TaqMan
5

Invitrogen™ RNA Control 250 with Manual  DFS Item

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

Content And Storage RNA Control and THE RNA Storage Solution should be stored at –20°C
Format Tube
Label or Dye Unconjugated
Product Line Ambion
6

Applied Biosystems™ QuantStudio™ 12K Flex OpenArray™ Calibration Kit  DFS Item

Offered in order to maintain the QuantStudio™ 12K Flex Real Time PCR System OpenArray™ block type.

7

Invitrogen™ Single Cell Lysis Kit  DFS Item
GREENER_CHOICE

Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.

8

Invitrogen™ Single Cell-to-CT™ qRT-PCR Kit  DFS Item
GREENER_CHOICE

The Ambion Single Cell-to-CT™ Kit contains a complete validated workflow for gene expression analysis for samples containing 1–10 cells.

Shipping Condition Dry Ice
Sample Type Mammalian Cells
Reaction Speed 7 min.
Purification Time 7 min.
Starting Material 1-10 Cells
For Use With (Application) RT-PCR
PCR Method 2-step RT-qPCR
Product Line Ambion, Cells-to-CT
Detection Method TaqMan
9

Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

Ambion™ Fast SYBR™ Green Cells-to-CT™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Shipping Condition Dry Ice
Content And Storage Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature.
Purity or Quality Grade UP (Ultra Pure)
Reverse Transcriptase M-MLV
Purification Time 5 min.
Concentration 20X
For Use With (Application) RT-PCR
Detection Method SYBR
Polymerase AmpliTaq Fast DNA Polymerase
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type Cells
Reaction Speed Fast
Label or Dye SYBR Green
Hot Start Built-In Hot Start
Product Line Cells-to-CT, SYBR
10

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification.

11

Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

12

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit  DFS Item
GREENER_CHOICE

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

13

Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer  DFS Item

These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.

14

Invitrogen™ Cells-to-CT Bulk RT Reagents  DFS Item
GREENER_CHOICE

Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).

15

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.