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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (649)
Standard PCR Products (423)
Reverse Transcription Products (100)
DNA Polymerases (69)
dNTPs (53)

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1 – 15 of 724 results
1
Promotions Available

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
3

Applied Biosystems™ PureQuant B Cell Assay  DFS Item

The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.

4

Applied Biosystems™ AmpliTaq Gold™ 360 DNA Polymerase  DFS Item
GREENER_CHOICE

The Applied Biosystems AmpliTaq Gold 360 DNA Polymerase, when used with improved AmpliTaq Gold 360 Buffer, and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Shipping Condition Room Temp or Wet Ice
GC-Rich PCR Performance High
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Hot-start PCR
Exonuclease Activity 5' - 3'
Polymerase AmpliTaq Gold 360 DNA Polymerase
Format Tube
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start Built-In Hot Start
Starting Material DNA
5

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

6

Applied Biosystems™ resDNASEQ™ Human Residual DNA Quantitation Kits
GREENER_CHOICE

The resDNASEQ Human Residual DNA Quantitation Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from human cell lines used as expression systems for recombinant proteins.

No. of Reactions 100 Reactions
Format Kit
Label or Dye FAM
Quantity 100 reactions
Host Cell Human DNA
PCR Method qPCR
Product Line resDNASEQ
Detection Method Primer-probe
8

Invitrogen™ DNA Polymerase I  DFS Item

DNA polymerase with 5'→3' and 3'→5' exodeoxyribonuclease activities

Shipping Condition Wet Ice
Polymerase DNA Polymerase I
Description DNA Polymerase
Hot Start No
Exonuclease Activity 3' - 5', 5' - 3'
9
Promotions Available

Applied Biosystems™ TaqMan™ Universal Master Mix II, with UNG  DFS Item

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

GC-Rich PCR Performance High
Content And Storage Store at 2°C to 8°C in the dark.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Fidelity (vs. Taq) 2 X
Purity or Quality Grade UP (Ultra Pure)
Concentration 2X
For Use With (Application) Gene Expression,Genotyping,miRNA analysis
PCR Method qPCR
Detection Method Primer-probe
Polymerase AmpliTaq Gold DNA Polymerase
Product Type Universal Master Mix II
Passive Reference Dye ROX (Pre-mixed)
For Use With (Equipment) 7300 System, 7500 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
Product Line TaqMan
10

Thermo Scientific™ Maxima Probe qPCR Master Mixes

Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.

11

Applied Biosystems™ GeneAmp™ 10X PCR Buffer  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer I is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Chemical Name or Material PCR Buffer
Concentration 10X
For Use With (Application) Standard PCR
12

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Polymerase AmpliTaq DNA Polymerase
Fidelity (vs. Taq) 1X
Reaction Format Kit
Product Type PCR Buffer
Concentration 10X
For Use With (Application) Standard PCR
13
Promotions Available

Applied Biosystems™ Absolute Q™ Liquid Biopsy dPCR Assay - NRAS 580  DFS Item

Absolute Q™ Liquid Biopsy dPCR assays enable reproducible, specific detection of known somatic mutations.