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Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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1

MilliporeSigma™ Immobilon Western HRP Chemiluminescence Substrates

Premixed, ready-to-use chemiluminescent reagents for detection of HRP-based Westerns

Product Type HRP Substrate
For Use With (Application) Dot/Slot Blot
3

MilliporeSigma™ Immobilon™ Western Chemiluminescent HRP Substrate (ECL)

Provides high sensitivity over a broad detection range at an economical price

Substrate Horseradish Peroxidase
Content And Storage 2°C to 8°C
Shelf Life 9 Months
For Use With (Application) Dot/Slot Blot
4

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Substrate Horseradish Peroxidase
Sensitivity Mid-femtogram
Form Liquid
Product Type Substrate
Signal Duration 24 hr.
Product Line SuperSignal
Detection Method Chemiluminescence
5

MilliporeSigma™ Luminata Crescendo ELISA HRP Substrate

Used on immunoblots as chemiluminescent substrate

Content And Storage 2°C to 8°C
6

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Content And Storage 2°C to 8°C
Kit Contents Detection reagent 1 and 2
Product Type ECL Western Blot Substrate
7

Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Content And Storage Upon receipt store at 4°C. Product shipped on ice.
Sensitivity Low picogram to mid-femtogram
Form Liquid
Product Type Substrate
Membrane Compatibility Nitrocellulose, PVDF
Product Line Pierce
Detection Method Chemiluminescence, Fluorescence
8

Thermo Scientific™ 1-Step™ NBT/BCIP Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

9

Thermo Scientific™ 1-Step™ TMB-Blotting Substrate Solution

Ready-to-use blue TMB peroxidase (HRP) substrate specially formulated for chromogenic detection in Western blot and IHC experiments.

10

Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

Thermo Scientific SuperSignal West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram to high femtogram-level protein detection by western blot analysis.

Shipping Condition Room Temperature
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:20-100 K (10-50 ng/mL), 1°: 1:1 K (0.2-1 μg/mL)
Conjugate HRP
Form Liquid
Product Type Substrate
Signal Duration 6 to 24 hr.
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence
11

Thermo Scientific™ 1-Step™ Ultra TMB-Blotting Solution

Thermo Scientific™ 1-Step Ultra TMB-Blotting Solution is an enhanced single-component horseradish peroxidase (HRP) substrate for Western blotting and immunohistochemistry.

12

Thermo Scientific™ Pierce™ ECL Western Blotting Substrate

Value-priced, entry-level peroxidase substrate for enhanced chemiluminescence (ECL) that directly replaces costlier products without the need to re-optimize conditions.

Substrate Horseradish Peroxidase
Content And Storage Upon receipt store at 2-8°C. Product is shipped at ambient temperature.
Form Liquid
Product Type Substrate
Signal Duration 1 to 2 hr.
Product Line Pierce
Detection Method Chemiluminescence
13

Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

14

Thermo Scientific™ SuperSignal™ West Femto Maximum Sensitivity Substrate

Ultra-sensitive enhanced chemiluminescent (ECL) HRP substrate for low-femtogram-level detection by Western blot analysis.

Substrate Horseradish Peroxidase
Form Liquid
Product Type Substrate
Signal Duration 6 to 8 hr.
Product Line SuperSignal
Detection Method Chemiluminescence
15

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.