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Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Catalog No. PI34000
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275 mL
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Catalog No. Quantity
PI34000 275 mL
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Catalog No. PI34000 Supplier Thermo Scientific™ Supplier No. 34000
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Description

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.

Thermo Scientific™ Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in blotting and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.

Highlights:

  • HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)

  • Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph

  • Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there is no dry powders to measure and dissolve

  • Stable – store refrigerated at 4°C for at least one year

Includes:

10X solution of CN/DAB and the Pierce Stable Peroxide Substrate Buffer

Specifications

Product Type Substrate
Detection Method Colorimetric
Form Liquid
Product Line Pierce
Quantity 275 mL
Conjugate HRP
Substrate Horseradish Peroxidase
Reactivity Horseradish Peroxidase
Sufficient For 25 Uses at 10 mL Each
Content And Storage Sufficient For: 25 uses at 10 mL each
• CN/DAB (10X), 25 mL
• Stable Peroxide Buffer, 250 mL

Store at 4°C.

For Research Use Only. Not for use in diagnostic procedures.