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Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution

Catalog No. PI34070
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PI34070 100 mL
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Catalog No. PI34070 Supplier Thermo Scientific™ Supplier No. 34070
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Description

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

Thermo Scientific™ Pierce NBT and BCIP Substrates are powders and ready-made solutions of nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate for chromogenic blot and IHC staining with alkaline phosphatase probes.

The combination of NBT (nitro-blue tetrazolium chloride) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) yields an intense, insoluble black-purple precipitate when reacted with alkaline phosphatase, a popular enzyme conjugate for antibody probes. Pierce NBT and BCIP powders are available individually and in two convenient, ready-to-use solutions (with and without a levamisole suppressor of endogenous phosphatase activity).

Highlights:

  • AP substrate – for detection of alkaline phosphatase (alk-phos) activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; produces purple-black precipitate that is easy to photograph

  • Package options – choose individual NBT and BCIP powders, or preformulated, ready-to-use solutions with or without levamisole suppressor

1-Step™ NBT/BCIP

  • Ideal for immunoblotting applications
  • Minimal assay-to-assay variability

1-Step NBT/BCIP Plus Suppressor

  • Ideal for staining tissues assayed with calf intestinal alkaline phosphatase without the interference caused by endogenous phosphatases
  • Contains 1mM levamisole to inhibit endogenous phosphatase activity

Specifications

Description Pierce 1-Step NBT/BCIP Plus Suppressor Substrate Solution
Product Type Substrate and Suppressor
Detection Method Colorimetric
Form Liquid
Membrane Compatibility Nitrocellulose, PVDF
Product Line 1-Step
Quantity 100 mL
Substrate Alkaline Phosphatase
Reactivity Alkaline Phosphatase
Content And Storage Upon receipt store at 4°C. Product is shipped with an ice pack.

Frequently Asked Questions (FAQs)

What color is the precipitate formed from the reaction of 1-Step NBT/BCIP plus Suppressor Substrate Solution and Alkaline Phosphatase?

The precipitate formed by the combination of NBT (nitro-blue tetrazolium) and BCIP (5-bromo-4-chloro-3'-indolyphosphate p-toluidine salt) is dark purple in color.
Why does the 1-Step NBT/BCIP plus Suppressor Substrate Solution contain 1 mM levamisole?

Levamisole is an inhibitor of Alkaline Phosphatase from non-calf intestinal sources. It will cut down on background signal from endogenous alkaline phosphatases.

Do I need to perform any weighing or measuring when I use 1-Step NBT/BCIP plus Suppressor Substrate Solution?

No; all of our “1-Step” Substrates are ready to use. Simply mix the bottle end over end to ensure homogeneity of the solution and it is ready to go.

For Research Use Only. Not for use in diagnostic procedures.